Protein Info for Pf6N2E2_4679 in Pseudomonas fluorescens FW300-N2E2
Annotation: Betaine aldehyde dehydrogenase (EC 1.2.1.8)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 95% identical to BETB_PSEF5: NAD/NADP-dependent betaine aldehyde dehydrogenase (betB) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)
KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a5247)MetaCyc: 76% identical to betaine aldehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Betaine-aldehyde dehydrogenase. [EC: 1.2.1.8]
Predicted SEED Role
"Betaine aldehyde dehydrogenase (EC 1.2.1.8)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.2.1.8)
MetaCyc Pathways
- choline-O-sulfate degradation (3/3 steps found)
- choline degradation I (2/2 steps found)
- glycine betaine biosynthesis I (Gram-negative bacteria) (2/2 steps found)
- glycine betaine biosynthesis II (Gram-positive bacteria) (2/2 steps found)
- glycine betaine biosynthesis III (plants) (2/3 steps found)
- β-alanine biosynthesis I (1/2 steps found)
- β-alanine biosynthesis IV (1/2 steps found)
- choline degradation IV (2/4 steps found)
- dimethylsulfoniopropanoate biosynthesis I (Wollastonia) (1/3 steps found)
- dimethylsulfoniopropanoate biosynthesis II (Spartina) (1/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.8
Use Curated BLAST to search for 1.2.1.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A299 at UniProt or InterPro
Protein Sequence (490 amino acids)
>Pf6N2E2_4679 Betaine aldehyde dehydrogenase (EC 1.2.1.8) (Pseudomonas fluorescens FW300-N2E2) MARFELQKLYIDGAYSDASGDATFEAINPANGEVLAQVQRATFEDVERAVVSAEKGQKVW AAMTAMQRSRILRRAVDILRERNDELAALETLDTGKAYSETRYVDIVTGTDVLEYYAGLV PAIEGEQIPLRDTSFVYTRREPLGVVAGIGAWNYPIQIALWKSAPALAAGNAMIFKPSEV TSLTTLKLAEIYTEAGVPPGVFNVLTGSGREVGTWLTEHPRIEKVSFTGGTDTGKKVMAS ASSSSLKDVTMELGGKSPLIIFDDADLDRAADTAMMANFYSSGQVCTNGTRVFVPSHLKA AFEAKIAERVARIRIGNPEDENTNFGPLVSFAHMESVLGYIEKGKAEGARLLCGGNRLTD GELAKGAFVAPTVFTDCTDEMTIVREEIFGPVMSILTYETEEEVIRRANDTDFGLAAGVV TRDLNRAHRVIHQLEAGICWINAWGESDAKMPVGGYKQSGVGRENGISSLNNFTRIKSVQ VELGDYASVF