Protein Info for Pf6N2E2_4473 in Pseudomonas fluorescens FW300-N2E2

Annotation: HlyD family secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 44 to 265 (222 residues), 95.9 bits, see alignment E=1.2e-31 PF16576: HlyD_D23" amino acids 48 to 266 (219 residues), 38.5 bits, see alignment E=1.2e-13 PF13533: Biotin_lipoyl_2" amino acids 51 to 97 (47 residues), 49.6 bits, see alignment 4e-17 PF13437: HlyD_3" amino acids 188 to 302 (115 residues), 46.1 bits, see alignment E=1.1e-15

Best Hits

Swiss-Prot: 43% identical to YHII_ECOLI: Uncharacterized protein YhiI (yhiI) from Escherichia coli (strain K12)

KEGG orthology group: K01993, HlyD family secretion protein (inferred from 99% identity to pba:PSEBR_a5438)

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A2X2 at UniProt or InterPro

Protein Sequence (321 amino acids)

>Pf6N2E2_4473 HlyD family secretion protein (Pseudomonas fluorescens FW300-N2E2)
MSTNHRTSRFFALALMTVLLAAGGFGYWKSRHDRLPEGLSMGNGRLEATEVQIASKTPGR
LAEVQVDEGDNVIKGQVLARMDTRTLEAQRNQAEAEVVRARQNLAATEANVQLRQSEQLL
AHQELKRTQELFKRGYASGQVIDQQQARVDTANAAVNAARAQVSAANAAIGATLAQVAQL
TSEIDDSTLRAPLDGVIQLRLAEPGEVLGAGGRVLLMIDPNDQYMNLYLPASVAGKLAVG
DEARLLLDALPERPLPAKVSFVAAKSQFTPKEVETRDERQKLVFRVKVRLTRPGEVPQAK
PGMPGAGYVRTAPIDWPANLQ