Protein Info for Pf6N2E2_4173 in Pseudomonas fluorescens FW300-N2E2

Annotation: Methionine ABC transporter permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 224 transmembrane" amino acids 24 to 47 (24 residues), see Phobius details amino acids 59 to 85 (27 residues), see Phobius details amino acids 94 to 114 (21 residues), see Phobius details amino acids 153 to 176 (24 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details PF00528: BPD_transp_1" amino acids 59 to 222 (164 residues), 63.4 bits, see alignment E=1.2e-21

Best Hits

Swiss-Prot: 47% identical to METP_BACSU: Methionine import system permease protein MetP (metP) from Bacillus subtilis (strain 168)

KEGG orthology group: K02072, D-methionine transport system permease protein (inferred from 100% identity to pba:PSEBR_a30)

MetaCyc: 52% identical to L-methionine/D-methionine ABC transporter membrane subunit (Escherichia coli K-12 substr. MG1655)
RXN0-4522 [EC: 7.4.2.11]; 7.4.2.11 [EC: 7.4.2.11]; TRANS-RXN-383 [EC: 7.4.2.11]; TRANS-RXN0-510 [EC: 7.4.2.11]; TRANS-RXN0-511 [EC: 7.4.2.11]

Predicted SEED Role

"Methionine ABC transporter permease protein" in subsystem Methionine Biosynthesis or Methionine Degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N7H207 at UniProt or InterPro

Protein Sequence (224 amino acids)

>Pf6N2E2_4173 Methionine ABC transporter permease protein (Pseudomonas fluorescens FW300-N2E2)
MELLSSFFSNIDWLEIWLATGDTFLMLFGSLLFTVLLGLPLGVLLFLCSPRQLLEARGVY
ALLSLIVNILRSLPFIILLIVMIPFTVLITGTSLGVAGAIPPLVVGATPFFARLVETALR
EVDRGIIEATQAMGATTRQIIVNALLPEARPGIFAAITVTAITLVSYTAMAGVVGAGGLG
DLAIRFGYQRFQTDVMVVTVVLLLVLVQVLQTVGDKLVVHFSRK