Protein Info for Pf6N2E2_4010 in Pseudomonas fluorescens FW300-N2E2

Annotation: Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 135 to 158 (24 residues), see Phobius details amino acids 164 to 180 (17 residues), see Phobius details PF00487: FA_desaturase" amino acids 11 to 223 (213 residues), 54.1 bits, see alignment E=2e-18 PF01610: DDE_Tnp_ISL3" amino acids 271 to 387 (117 residues), 37.8 bits, see alignment E=2e-13

Best Hits

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a178)

Predicted SEED Role

"Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1)" (EC 1.14.19.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.19.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZZ09 at UniProt or InterPro

Protein Sequence (394 amino acids)

>Pf6N2E2_4010 Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1) (Pseudomonas fluorescens FW300-N2E2)
MWYEGFLGLSAWSLVAVTLLMTHVTIIAVTVYLHRYSAHRSLELNAGLKHFFRFWLWLTT
AQNTREWTAIHRKHHAKCETEDDPHSPVIKGLSTVLRKGAELYRAEAENPETLRIYGKNC
PDDWIERNLYSRFPLLGVAIMGIIDLLLFGTIGITIWAIQMMWIPVWAAGVVNGLGHAVG
YRNFECRDAATNLVPWGILIGGEELHNNHHTYPNSAKLSVRKWEFDLGWAWIRVFSILRL
AKVQRVAPIAHRVEGKGHLDMDTAMAILNNRFQIMAQYRRLVIAPLVKQELEKVDHSVRH
QFHRAKRLLSRETSLLDDRHHVRIQNMLEHSQALKVIYEKRLALQQIWVKTSSNGHDMLA
AIKEWVHEAEASGIQSLREFADQLKTYSLRPATA