Protein Info for Pf6N2E2_3997 in Pseudomonas fluorescens FW300-N2E2

Annotation: transcriptional regulator, Crp/Fnr family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 PF00027: cNMP_binding" amino acids 34 to 120 (87 residues), 68.3 bits, see alignment E=4.8e-23 PF13545: HTH_Crp_2" amino acids 153 to 223 (71 residues), 57.7 bits, see alignment E=9.2e-20

Best Hits

KEGG orthology group: None (inferred from 92% identity to pba:PSEBR_a190)

Predicted SEED Role

"transcriptional regulator, Crp/Fnr family" in subsystem Oxidative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZIM0 at UniProt or InterPro

Protein Sequence (228 amino acids)

>Pf6N2E2_3997 transcriptional regulator, Crp/Fnr family (Pseudomonas fluorescens FW300-N2E2)
MNSHPWHSQLMAGHWYSHLPVELQNSLLDMARERRLPPGHGLFKRGDPPCGLYAVLEGAV
RIGAVNEQGKEALLSMIEAPHWFGEICLFDGQPRTHDAVVVGQGSLLHVPQAPLLAFLQA
QPIYWRHLALLMSHKLRLTFINLEHLSLMPAPARVAHRLLMIAEGYGEIEPPRRILQLPQ
EHLALMLALSRQTTNQILKDLQAQGILHLGYGEIEILDIERLRGLTTI