Protein Info for Pf6N2E2_3845 in Pseudomonas fluorescens FW300-N2E2

Annotation: A/G-specific adenine glycosylase (EC 3.2.2.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 743 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF05170: AsmA" amino acids 1 to 629 (629 residues), 383.5 bits, see alignment E=2.4e-118 PF13502: AsmA_2" amino acids 476 to 659 (184 residues), 80.8 bits, see alignment E=1.2e-26

Best Hits

KEGG orthology group: K07289, AsmA protein (inferred from 98% identity to pba:PSEBR_a341)

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZYJ9 at UniProt or InterPro

Protein Sequence (743 amino acids)

>Pf6N2E2_3845 A/G-specific adenine glycosylase (EC 3.2.2.-) (Pseudomonas fluorescens FW300-N2E2)
MKAFGKILGLVLLGLLLIIVALGFALTHLFDPNDYKEEIRQIARDKAHIELTLNGDIGWS
LFPWLGLELHDASVATLASPTQPFADLQMLGLSVRVIPLLRREVQMSDVRVEGLNLRLNR
DKNGHGNWEDIGKVPASASTANAPAPVPSQQPTPPVSTPAEKPAQPTRLDIDSLTVNNAR
VEYTDEQTGKLFTAESIQLSTGPVHDSTNIPVTLTAFLSSNQPAVRVRTEVTGELRFERA
LKRYKFEDLKLSGEATGDPLQGKTLNFAIQGQLFLDKAANVAEWTGIKLSLNQLRALGEL
KVNDLDKTPQLNGGVSIAQFDLAKFVESIGQKLPAMAEGSLSKVELSSRIAGTPTSVELN
DINLKVDDSNFSGRIAVEDFAKQSLRAQLKADTFNVDRYLEPKSAEADSSKAAREAEVSN
TEASAMAGAGSTPLPSAPTQGAWSNDRLFPVERLSKLDLDADLAFGQLTLDKLPIQNAAL
KATGRGGLLTLENLRGDLYSGNFETKGTLDVRQPTPQLSLQTRINRVPAEKIIESQGKNP
PVKGLVTVNSAITASGNSQKALIDSLNGTAGFVINDGVLLNANLEQQLCKGIATLNRKSL
SGDPRGKDTPFQQLNGNLTFNNGVANNPDLKVRIPGMTVNGNGDIDLRVLGMDYRVGVIV
EGDKSDMPDPACQVNERYVDVEWPLRCRGPLELGAKACRLDNDGLGKVAAKLAGDRLGDK
IDEKLGDKVSPELKDALKGLFKR