Protein Info for Pf6N2E2_3767 in Pseudomonas fluorescens FW300-N2E2

Annotation: Arginyl-tRNA synthetase (EC 6.1.1.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 PF03485: Arg_tRNA_synt_N" amino acids 4 to 90 (87 residues), 88.6 bits, see alignment E=5e-29 TIGR00456: arginine--tRNA ligase" amino acids 6 to 578 (573 residues), 556.3 bits, see alignment E=3.5e-171 PF00750: tRNA-synt_1d" amino acids 101 to 448 (348 residues), 419.5 bits, see alignment E=1.5e-129 PF05746: DALR_1" amino acids 462 to 578 (117 residues), 118.6 bits, see alignment E=2.5e-38

Best Hits

Swiss-Prot: 92% identical to SYR_PSEPF: Arginine--tRNA ligase (argS) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 92% identity to pfo:Pfl01_0399)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A2C1 at UniProt or InterPro

Protein Sequence (578 amino acids)

>Pf6N2E2_3767 Arginyl-tRNA synthetase (EC 6.1.1.19) (Pseudomonas fluorescens FW300-N2E2)
MKDTIRQLIQQAIAQLVTEGVLPEGLSPAIQVENTRDKTHGDFASNIAMMLAKPAGMKPR
DLAEKIIAALPADENVSKADIAGPGFINFFQNTQALASRLDAALADERIGVRKAGPLQRT
VVDLSAPNLAKEMHVGHLRSTIIGDGVARVLEFLGDTVIRQNHVGDWGTQFGMLMAYLQE
NPITSDELSDLENFYRAAKQRFDESPEFADRARGLVVKLQAGDAECLALWSKFKDISLSH
CQKIYELLNVKLTMADVMGESAYNDDLINVVNDLKAKGMLVESNGAQCVFLDQFKTADGE
PLPVIIVKADGGYLYATTDLAAVRYRNSVLKADRVLYFVDQRQALHFQQVFEVARLAGFV
THPMEMEHMGFGTMNGADGRPFKTRDGGTVKLIDLLTEAQDRAYTLVKGKNPDLAEDELR
NIAKVVGIDAVKYADLSKHRTSDYSFNFDLMLNFEGNTAPYLLYAYTRVAGVFRKLGKSF
NEVDGQIVLEAPHEQELAAKLAQFGEVLNNVADKGTPHTLCAYLYDVAGLFSSFYENCPI
LNAQTPEQMQSRLRLAALTGRTLKQGLELLGLETLERM