Protein Info for Pf6N2E2_3759 in Pseudomonas fluorescens FW300-N2E2

Annotation: Type IV pilus biogenesis protein PilN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 188 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details PF05137: PilN" amino acids 100 to 177 (78 residues), 78.9 bits, see alignment E=1.2e-26

Best Hits

KEGG orthology group: K02663, type IV pilus assembly protein PilN (inferred from 97% identity to pba:PSEBR_a423)

Predicted SEED Role

"Type IV pilus biogenesis protein PilN" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZYH6 at UniProt or InterPro

Protein Sequence (188 amino acids)

>Pf6N2E2_3759 Type IV pilus biogenesis protein PilN (Pseudomonas fluorescens FW300-N2E2)
MARINLLPWREELREERRKRFLLALVGALVGAVGVVLVAVRYFDSAIDHQVARNSHLSEQ
IAVLDERIKQISELKARRQQLVERMRIIQDLQGNRPVSGRIFDQLVRTLPDGVYFTEVKM
EDRTLLIKGAAESNNRVSDLMRNLDSSDLFDTPSLTEVKATTAGQLDQANVFQLTVRQTR
PADAEDAQ