Protein Info for Pf6N2E2_3644 in Pseudomonas fluorescens FW300-N2E2

Annotation: Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 629 transmembrane" amino acids 473 to 491 (19 residues), see Phobius details PF13667: ThiC-associated" amino acids 24 to 101 (78 residues), 109.8 bits, see alignment E=4.6e-36 TIGR00190: phosphomethylpyrimidine synthase" amino acids 140 to 592 (453 residues), 720.9 bits, see alignment E=2.3e-221 PF01964: ThiC_Rad_SAM" amino acids 142 to 590 (449 residues), 615.6 bits, see alignment E=4.4e-189

Best Hits

Swiss-Prot: 96% identical to THIC_PSEPF: Phosphomethylpyrimidine synthase (thiC) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 99% identity to pba:PSEBR_a512)

MetaCyc: 73% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A042 at UniProt or InterPro

Protein Sequence (629 amino acids)

>Pf6N2E2_3644 Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17) (Pseudomonas fluorescens FW300-N2E2)
MTTKSKNATNLSDSAKVDEQSVQPFTRSQKIYVQGSRPDIRVPMREISLDVTPTDFGGEI
NAPVVVYDTSGPYTDPNVIIDVRKGLADVRSSWIEARGDTERLAGLSSNFGQERLADPEL
TKLRFAHVNNPRRAKPGANVSQMHYARKGIITAEMEYVAIRENMKLEVARAAGLLDQQHA
GHSFGASVPKVITPEFVRDEIARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALG
SSIEEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVGGV
AEDLTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAH
HKENFLYTHFEDICEIMKAYDVSFSLGDGLRPGSIADANDEAQFGELETLGELTKIAWKH
DVQCMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIG
WFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFE
FRWEDQFNLGLDPDTARSYHDETLPKDSAKVAHFCSMCGPKFCSMKITQEVREYAANQRI
EAVDVDVAQGLAEQAERFKKEGSQLYKKV