Protein Info for Pf6N2E2_3585 in Pseudomonas fluorescens FW300-N2E2
Annotation: Nicotinate phosphoribosyltransferase (EC 2.4.2.11)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to PNCB_PSEPF: Nicotinate phosphoribosyltransferase (pncB) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K00763, nicotinate phosphoribosyltransferase [EC: 2.4.2.11] (inferred from 100% identity to pba:PSEBR_a566)MetaCyc: 53% identical to nicotinate phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
NICOTINATEPRIBOSYLTRANS-RXN [EC: 6.3.4.21]
Predicted SEED Role
"Nicotinate phosphoribosyltransferase (EC 2.4.2.11)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation or Redox-dependent regulation of nucleus processes (EC 2.4.2.11)
MetaCyc Pathways
- NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
- NAD salvage pathway V (PNC V cycle) (3/5 steps found)
- NAD salvage (plants) (5/11 steps found)
- superpathway of NAD biosynthesis in eukaryotes (6/14 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Nicotinate and nicotinamide metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.4.2.11, 6.3.4.21
Use Curated BLAST to search for 2.4.2.11 or 6.3.4.21
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZY47 at UniProt or InterPro
Protein Sequence (408 amino acids)
>Pf6N2E2_3585 Nicotinate phosphoribosyltransferase (EC 2.4.2.11) (Pseudomonas fluorescens FW300-N2E2) MSESVFADRIVQNLLDTDFYKLTMMQAVLHNYPNVEVEWEFRCRNSEDLRPYLAEIRFQI ERLAELSLSADQLGFLERISFLKPDFLRFLGLFRFNLRYVHTGIENGELFIRLRGPWLHV ILFEVPLLAIVSEVRNRYRYREIVLEQAREQLYRKFDWLSANASAEELSELQVADFGTRR RFSFRVQEEVVNVLKHDFPGRFVGTSNVHLSRELDMKPLGTMAHEWIMAHQQLGPRLIDS QIAALDCWVREYRGLLGIALTDCITMDAFLKDFDLFFAKLFDGLRHDSGDPVIWAEKAIA HYHKLGIDPMSKTLVFSDSLTLPKCLEIFRALRGRINVSFGIGTNLTCDIPGVEPMSIVL KMISCDGQPVAKISDEPGKTHCKDPNFVAYMRHVFQVPAALSDTSSKE