Protein Info for Pf6N2E2_3234 in Pseudomonas fluorescens FW300-N2E2

Annotation: mandelate racemase/muconate lactonizing enzyme family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 353 PF02746: MR_MLE_N" amino acids 4 to 94 (91 residues), 39.4 bits, see alignment E=6.2e-14 PF13378: MR_MLE_C" amino acids 123 to 341 (219 residues), 170.7 bits, see alignment E=3.8e-54

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a880)

Predicted SEED Role

"mandelate racemase/muconate lactonizing enzyme family protein" in subsystem Catechol branch of beta-ketoadipate pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZZ99 at UniProt or InterPro

Protein Sequence (353 amino acids)

>Pf6N2E2_3234 mandelate racemase/muconate lactonizing enzyme family protein (Pseudomonas fluorescens FW300-N2E2)
MIKMALLRIGCDDGSEGYAFGPPELIRPHIIESFVRKVLIGRDPMDRESLWQDLAHWQRG
SAGQFTDRALALVEQALWDLAGRALKLPVHKLIGGYRDKVPAYGSTMCGDDLPGGLSTPD
EYGQFAEKLVQRGYKAIKLHTWMPPISFAPNPQMDIQACAAVREAVGPDIALMLDGYHWY
SRMDALTIGKALQKLNFAWFEEPMMEDSAESYAWLAANLDIPVLGPESIAGKFHSRASWV
TQKSCDILRAGVAGVGGIGPCLKVAHLAESFGMDCEVHGNGAANLAVVGAISNCRWYERG
LLHPFLDYEEVPAHLNSIVDPMDADGFVHLSDRPGLGEDINFAYIEANTLSRH