Protein Info for Pf6N2E2_320 in Pseudomonas fluorescens FW300-N2E2

Annotation: TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 675 transmembrane" amino acids 18 to 36 (19 residues), see Phobius details amino acids 42 to 60 (19 residues), see Phobius details amino acids 72 to 89 (18 residues), see Phobius details amino acids 101 to 118 (18 residues), see Phobius details amino acids 125 to 144 (20 residues), see Phobius details amino acids 174 to 192 (19 residues), see Phobius details amino acids 272 to 292 (21 residues), see Phobius details amino acids 298 to 316 (19 residues), see Phobius details amino acids 337 to 356 (20 residues), see Phobius details amino acids 362 to 383 (22 residues), see Phobius details amino acids 395 to 428 (34 residues), see Phobius details amino acids 448 to 479 (32 residues), see Phobius details amino acids 486 to 507 (22 residues), see Phobius details amino acids 510 to 512 (3 residues), see Phobius details amino acids 528 to 529 (2 residues), see Phobius details amino acids 533 to 556 (24 residues), see Phobius details amino acids 566 to 586 (21 residues), see Phobius details amino acids 598 to 617 (20 residues), see Phobius details amino acids 628 to 646 (19 residues), see Phobius details amino acids 651 to 667 (17 residues), see Phobius details TIGR02123: TRAP transporter, 4TM/12TM fusion protein" amino acids 16 to 669 (654 residues), 761.4 bits, see alignment E=4.1e-233 PF06808: DctM" amino acids 112 to 594 (483 residues), 280.3 bits, see alignment E=1.3e-87

Best Hits

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a3539)

Predicted SEED Role

"TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YRY4 at UniProt or InterPro

Protein Sequence (675 amino acids)

>Pf6N2E2_320 TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1 (Pseudomonas fluorescens FW300-N2E2)
MSEDQHGIATDPRDWPKTLFYVALLFSIFQIVTAAFSPVSSIVLRAVHVGFLLWVVFLSY
PAHGRQRPWQPLAWVLSLGGVATALYQWVFESDLIQRSGDLTTTDMIMGIVLVVLVFEAA
RRVMGIALPIICGLFLAYGLFGEYLPGDLAHRGYGLDQIINQLSFGTEGLYGTPTYVSAT
YIFLFILFGAFLEKAGMIKLFTDFAMGLFGHKLGGPAKVAVVSSALMGTITGSGIANVVT
TGQFTIPLMKRFGYKAAFAGGVEATSSMGSQIMPPVMGAVAFIMAETINVPFVEIAKAAL
IPACLYFGSVFWMVHLEAKRSDLKGLPKDQCPSAWGAVKDSWFLLIPLGVLVYLLFSGRT
PLFSGMVGLALTAIVILGSAIILKVSNYALRCAFWIALGLLCVGFFRLGIGVVFAVIGVL
VVACWFMQGTRETLVICLHALVDGARHAVPVGIACALVGSIIAVVSLTGVASTFAGYILA
IGRDNLLLSLILTMLTCLVLGMGIPTIPNYIITSSIAAPALLELGVPLIVSHMFVFYFGI
LADLTPPVALACFAAAPIARESGLKISFWAVRIALAGFVIPFMTVYNPALMLQGDNLWMT
AYMLIKTLLAVGLWGMASTGYLQQKMPIWERLLCFAAGALLVVALPVTDEIGFVLGGLLI
LQHVWRARRSGRALA