Protein Info for Pf6N2E2_2781 in Pseudomonas fluorescens FW300-N2E2

Annotation: Urea carboxylase (EC 6.3.4.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1226 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 143.8 bits, see alignment E=1.1e-45 TIGR02712: urea carboxylase" amino acids 2 to 1224 (1223 residues), 1911.4 bits, see alignment E=0 PF02786: CPSase_L_D2" amino acids 116 to 319 (204 residues), 205.6 bits, see alignment E=2.7e-64 PF07478: Dala_Dala_lig_C" amino acids 147 to 291 (145 residues), 41.8 bits, see alignment E=3.6e-14 PF02785: Biotin_carb_C" amino acids 338 to 446 (109 residues), 104 bits, see alignment E=1.9e-33 TIGR00724: biotin-dependent carboxylase uncharacterized domain" amino acids 454 to 759 (306 residues), 279.6 bits, see alignment E=2.9e-87 PF02626: CT_A_B" amino acids 478 to 750 (273 residues), 309.2 bits, see alignment E=1.1e-95 PF02682: CT_C_D" amino acids 828 to 1047 (220 residues), 141.2 bits, see alignment E=1.5e-44 PF00364: Biotin_lipoyl" amino acids 1164 to 1223 (60 residues), 53.9 bits, see alignment 5.2e-18

Best Hits

Predicted SEED Role

"Urea carboxylase (EC 6.3.4.6)" in subsystem Urea decomposition (EC 6.3.4.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A094 at UniProt or InterPro

Protein Sequence (1226 amino acids)

>Pf6N2E2_2781 Urea carboxylase (EC 6.3.4.6) (Pseudomonas fluorescens FW300-N2E2)
MFEKILIANRGAIACRILRTLRELKVQGVAVYSQADAASLHILQADEAHCLGEGAAAGTY
LAVDKLLAIAKGSGATAIHPGYGFLSENAAFAEACEAAGIAFIGPTPEQLRVFGLKHTAR
DLARQHGVPLLEGTELLDSLDAALLAATRIGYPVMLKSTAGGGGIGMRVCRSAAELSESF
EAVKRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGQGQVIALGVRDCSVQRRNQKVLEE
TPAPNLPEGMADELCAAAIQLAQAVNYRSAGTVEFVFDSDAGRFYFLEVNTRLQVEHGVT
EQVWGVDLVRWMVQLAAGDLPPLNELGQGLKAEGHAIQARLYAEDPGRDFQPSPGLLTAV
KFPQADGKALRIDTWVEAGCQIPPYFDPMIAKVIRWAPTREQARLGLYQALDESLLYGVE
TNRQYLQQILLDTPFASGQPWTRCLEALVYRANTVEVLSPGTQTSVQDYPGRLGYWAVGV
PPSGPMDSRSLRLGNRLLGNEEGAAALEITMNGPLLRFNCAARVTVTGAVIALTLDGEAV
PMNTPLSIAAGATLAIGNISGAGARSYLCLQGGVQVPDYLGSKSTFTLGQFGGHGGRALC
TGDVLHLTPLDEHTTLPPTSAPILELPSVRQIRVIYGPHGAPEYFTERYIQTFFDTAWEV
HFNSSRTGVRLIGPKPEWVRADGGEAGLHPSNIHDNPYAIGAVDFTGDMPVILGPDGPSL
GGFVCPVTVIEADLWQLGQLKAGDKVRFVPVDISTARSLATHIPCGEGACSRSNAQHSPD
NGPTDKELGPLRDPARASSLATGSGCTQKWGSPVVLDIGQDDTRLVARLSGDTHLLLEIG
APELDLVLRFRAHALMQALEHKHLPGVIDLTPGIRSLQVHYQPEQLPLADLLSIVAGEWD
AVCAAQDLQVPSRIVHLPLSWDDPACQLAIEKYMTTVRKDAPWCPSNLEFIRRINDLPNL
DEVQRTVFDASYLVMGLGDVYLGAPVATPLDPRHRLVTTKYNPARTWTAENSVGIGGAYM
CVYGMEGPGGYQFVGRTLQMWNRYRDVAAFDGKPWLLRFFDQIRFYPVSADELLRIRRDF
PLGRFDLAIEHSQLNLAEYQRFLAQEADSIAAFRHQQQRAFEAERERWIASGQAHFDSEE
PAIAPSEDSPLTDGQLSVDSHIAGNLWQVQVEVGARVAAGDVLVILESMKMEIPVLAPVA
GVVREVRVQPGSAVRAGQRVVVLERD