Protein Info for Pf6N2E2_269 in Pseudomonas fluorescens FW300-N2E2
Annotation: NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 76% identical to NUOJ_PSEAE: NADH-quinone oxidoreductase subunit J (nuoJ) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00339, NADH dehydrogenase I subunit J [EC: 1.6.5.3] (inferred from 99% identity to pba:PSEBR_a3589)MetaCyc: 89% identical to NADH-quinone oxidoreductase subunit J (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]
Predicted SEED Role
"NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)
MetaCyc Pathways
- aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
- NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
- aerobic respiration I (cytochrome c) (3/4 steps found)
- NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
- Fe(II) oxidation (3/6 steps found)
- NAD(P)/NADPH interconversion (3/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.6.5.3
Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165YQ01 at UniProt or InterPro
Protein Sequence (166 amino acids)
>Pf6N2E2_269 NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3) (Pseudomonas fluorescens FW300-N2E2) MEFAFYFASGIAVVSTLRVVTNTNPIHALLYLIISLIAVAMTFFALGAPFAGALEVIAYA GAIMVLFVFVVMMLNLGPAAVQQERSWLKPGIWAGPVILSALLLGELLYVLFAHQSGQAI GHTTVGAKAVGISLFGPYLLVVELASMLLLAAAVTAFHLGRNEAKE