Protein Info for Pf6N2E2_265 in Pseudomonas fluorescens FW300-N2E2

Annotation: NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 transmembrane" amino acids 6 to 30 (25 residues), see Phobius details amino acids 37 to 56 (20 residues), see Phobius details amino acids 75 to 96 (22 residues), see Phobius details amino acids 108 to 126 (19 residues), see Phobius details amino acids 132 to 152 (21 residues), see Phobius details amino acids 164 to 183 (20 residues), see Phobius details amino acids 206 to 230 (25 residues), see Phobius details amino acids 242 to 262 (21 residues), see Phobius details amino acids 272 to 294 (23 residues), see Phobius details amino acids 301 to 321 (21 residues), see Phobius details amino acids 330 to 351 (22 residues), see Phobius details amino acids 373 to 398 (26 residues), see Phobius details amino acids 410 to 436 (27 residues), see Phobius details amino acids 456 to 473 (18 residues), see Phobius details TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N" amino acids 8 to 476 (469 residues), 421.6 bits, see alignment E=2.3e-130 PF00361: Proton_antipo_M" amino acids 126 to 422 (297 residues), 269.5 bits, see alignment E=1.7e-84

Best Hits

Swiss-Prot: 78% identical to NUON_PSEAE: NADH-quinone oxidoreductase subunit N (nuoN) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 100% identity to pba:PSEBR_a3593)

MetaCyc: 87% identical to NADH-quinone oxidoreductase subunit N (Pseudomonas putida KT2440)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YPW1 at UniProt or InterPro

Protein Sequence (487 amino acids)

>Pf6N2E2_265 NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3) (Pseudomonas fluorescens FW300-N2E2)
MEFTIQHFIALAPLLITSATIIVVMLAIAWRRNHSQTFLLSVAGLNLALLSILPALKVAP
LAVTPLMQIDSFACLYMALILVATLACVTLAHAYLGDGGSGYPGNREELYLLILMAAAGG
LVLVSAQHLASLFIGLELLSVPVYGLVAYAFFNKRSLEAGIKYMVLSAAGSAFLLFGMAL
LYAEAGTLSFVGIGQALAATGLPSPIAQLGLGMMLIGLAFKLSLVPFHLWTPDVYEGAPA
PVAAFLATASKVAVFAVMVRLFQISPVASSGVLSNVLTIIAIASILFGNLLALTQSNLKR
LLGYSSIAHFGYLLIALIASKGLAVEAIGVYLVTYVITSLGAFGVITLMSSPYNGRDADA
LYEYRGLFWRRPYLTAVLTVMMLSLAGIPLTAGFIGKFYIIATGVEAHQWWLVGSLVLGS
AIGVFYYLRVMVTLYLMEPNLRRHDAELHWEQKAGGVMLLAIAVLAFFLGVYPQPLLTLV
QQAGLTG