Protein Info for Pf6N2E2_2541 in Pseudomonas fluorescens FW300-N2E2

Annotation: 5-methylaminomethyl-2-thiouridine-forming enzyme mnmC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 660 PF05430: Methyltransf_30" amino acids 115 to 234 (120 residues), 135.8 bits, see alignment E=1.1e-43 PF01266: DAO" amino acids 264 to 629 (366 residues), 171.9 bits, see alignment E=4.3e-54 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 264 to 658 (395 residues), 448.3 bits, see alignment E=1.3e-138 PF13450: NAD_binding_8" amino acids 266 to 297 (32 residues), 26.1 bits, see alignment (E = 1.3e-09)

Best Hits

Swiss-Prot: 82% identical to MNMC_PSEF5: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a1528)

Predicted SEED Role

"5-methylaminomethyl-2-thiouridine-forming enzyme mnmC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165Z9C7 at UniProt or InterPro

Protein Sequence (660 amino acids)

>Pf6N2E2_2541 5-methylaminomethyl-2-thiouridine-forming enzyme mnmC (Pseudomonas fluorescens FW300-N2E2)
MTPVQHHAQLDWDDQGRPRSRVFDDVYFSDQSGLEETRYVFLEQNNLAERFAALADDGRL
VIGETGFGTGLNFLCAWQLFEQCAPAGARLHFVSVEKYPLTPQDLCRALALWPQLALQAE
QLLGQYVAIHQGFQRLVLAGGRVTLTLLIGDALEQLPQLDGQIDAWFLDGFAPARNPEMW
TAELFAELARLAAPGATLSTFTSTGWVRRLLNAAGFKMKRTPGIGHKWEILRGVFLGWPE
QTPAPASAKPWYARPLPPIGERRALVIGGGLAGCASAASLAARGWQVTLLERHAGLAEEA
SGNPQGVLYLKLSAHGTALSQMILSGFGYTRRVLEQLQRGIDWDACGVLQLAFNSKEAER
QAQLAEAFPRDLLHPLDQSEAQVRSGIGLACGGLFYPEGGWVHPPALCRWQASGTAIDVQ
PHHDVLQLRRVDGQWQAWDGERCLASAPVAVLASAAEIKRFEPAADLPLKRIRGQITRLP
QTTRSLSLATVVCAEGYVAPPRLGEHTLGASFDFNSDDLTPTAAEHAGNLQMLEEISQDL
VDRLNAGSLDPERLEGRAAFRCTSPDYLPIVGPLADRQAFSEAYSALGKDARQVPDTSCP
WFEGLYINSGHGSRGLITAPLSGELIAAWLDNEPLPLPRSVAEACHPNRFALRALIRGKA