Protein Info for Pf6N2E2_2513 in Pseudomonas fluorescens FW300-N2E2
Annotation: UDP-glucose dehydrogenase (EC 1.1.1.22)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 68% identical to VIPA_SALTI: Vi polysaccharide biosynthesis protein VipA/TviB (vipA) from Salmonella typhi
KEGG orthology group: K02474, UDP-N-acetyl-D-galactosamine dehydrogenase [EC: 1.1.1.-] (inferred from 78% identity to bav:BAV0511)MetaCyc: 77% identical to UDP-N-acetylgalactosamine 6-dehydrogenase (Pseudomonas aeruginosa)
UDP-N-acetylglucosamine 6-dehydrogenase. [EC: 1.1.1.136]; 1.1.1.136 [EC: 1.1.1.136]
Predicted SEED Role
"UDP-glucose dehydrogenase (EC 1.1.1.22)" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance ) or Teichuronic acid biosynthesis (EC 1.1.1.22)
MetaCyc Pathways
- colanic acid building blocks biosynthesis (9/11 steps found)
- UDP-N-acetyl-α-D-galactosaminuronate biosynthesis (2/2 steps found)
- UDP-α-D-glucuronate biosynthesis (from UDP-glucose) (1/1 steps found)
- superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
- UDP-α-D-xylose biosynthesis (1/2 steps found)
- UDP-2,3-diacetamido-2,3-dideoxy-α-D-mannuronate biosynthesis (1/5 steps found)
- UDP-sugars interconversion (2/9 steps found)
- teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
- superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (10/24 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of type II polyketide products
- Biosynthesis of unsaturated fatty acids
- Bisphenol A degradation
- Butanoate metabolism
- C21-Steroid hormone metabolism
- Fructose and mannose metabolism
- Glycine, serine and threonine metabolism
- Insect hormone biosynthesis
- Linoleic acid metabolism
- Nucleotide sugars metabolism
- Pentose and glucuronate interconversions
- Polyketide sugar unit biosynthesis
- Retinol metabolism
- Starch and sucrose metabolism
- Tetrachloroethene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.22
Use Curated BLAST to search for 1.1.1.- or 1.1.1.136 or 1.1.1.22
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZXJ0 at UniProt or InterPro
Protein Sequence (427 amino acids)
>Pf6N2E2_2513 UDP-glucose dehydrogenase (EC 1.1.1.22) (Pseudomonas fluorescens FW300-N2E2) LNNQDFENVKLGIVGLGYVGLPLAVEFGKHYSVLGFDISERRIEELSGGRDSTLEVSEQE LGEAKNLQFSTSVEKLSNCNIFIVTVPTPIDQYNNPDLTPLLKASETIGSVLKKGDVVIY ESTVYPGATEEDCVPVLERVSGLKFNSDFYVGYSPERINPGDKEHRVTTIKKVTSGSTPE VATKIDNLYKKIIIAGTHKASSIKVAEAAKVIENTQRDLNIALINELAIIFNKLGIDTES VLKAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQSIGYHPEIILAGRRLNDSMGEYVV SQLVKAMVKKRIHVDGARVLIMGLTFKENCPDLRNTRVIDIISELADYNIVVDVFDPWAA AEEAEHEYGIKLLSTPAPDSYDAIVLAVAHSQFADMGSRHIRSLGKANHVLYDLKYLLDN DESDIRL