Protein Info for Pf6N2E2_2510 in Pseudomonas fluorescens FW300-N2E2

Annotation: Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 376 transmembrane" amino acids 62 to 80 (19 residues), see Phobius details amino acids 100 to 122 (23 residues), see Phobius details PF13477: Glyco_trans_4_2" amino acids 4 to 131 (128 residues), 43.3 bits, see alignment E=1e-14 PF20706: GT4-conflict" amino acids 180 to 312 (133 residues), 34.6 bits, see alignment E=2.8e-12 PF00534: Glycos_transf_1" amino acids 194 to 351 (158 residues), 135.3 bits, see alignment E=4e-43 PF13692: Glyco_trans_1_4" amino acids 196 to 337 (142 residues), 120.3 bits, see alignment E=2e-38 PF13524: Glyco_trans_1_2" amino acids 255 to 362 (108 residues), 35.4 bits, see alignment E=2.4e-12

Best Hits

KEGG orthology group: None (inferred from 54% identity to pag:PLES_19151)

MetaCyc: 33% identical to UDP-GalNAc:alpha-D-GlcNAc-PP-Und alpha-(1,3)-GalNACtransferase (Salmonella enterica enterica serovar Poona)
2.4.1.-

Predicted SEED Role

"Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase" in subsystem N-linked Glycosylation in Bacteria (EC 2.4.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-

Use Curated BLAST to search for 2.4.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZZP9 at UniProt or InterPro

Protein Sequence (376 amino acids)

>Pf6N2E2_2510 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase (Pseudomonas fluorescens FW300-N2E2)
MSRILLIVVNDPRYFLSHRLPIAKAAQGKGYAVHVASIASPLSGKVIEEGFIYHTIKLER
GGINPLLEFFAMISFVRLLWRLRPTLLHLVTTKPVLYGGMAARLAPVGGVVSAIAGMGSV
FIGRDLRSKVLSFVLSRMYRLALGAHNLRAIFQNPCDRALVMKMSGLDADKTVLIRGSGV
HLASYPVLPEPIGTITVTFAGRFLRDKGVHEFIKAAQLVKRRQNDVVFQLVGDVDEGNPT
SLSSTEVEALTVEGNVRVLGYQSDLAKIFSRSHIVVLPSYREGLPKVLIEAAACARPVVT
SDVPGCRDAIIAGETGLLVPVKDAEALAIAIEKLVSNAELRHEMGRAGRKWAEREFAIEN
VVKVHLDVYQALEAET