Protein Info for Pf6N2E2_2409 in Pseudomonas fluorescens FW300-N2E2

Annotation: Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 538 PF01039: Carboxyl_trans" amino acids 49 to 519 (471 residues), 403.6 bits, see alignment E=1.2e-124 PF03255: ACCA" amino acids 92 to 204 (113 residues), 34.2 bits, see alignment E=1.7e-12 amino acids 340 to 419 (80 residues), 29.9 bits, see alignment E=3.4e-11

Best Hits

Swiss-Prot: 47% identical to MCCB_HUMAN: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2) from Homo sapiens

KEGG orthology group: K13778, geranyl-CoA carboxylase beta subunit [EC: 6.4.1.5] (inferred from 98% identity to pba:PSEBR_a1661)

MetaCyc: 82% identical to cis-geranyl-CoA carboxylase beta-subunit (Pseudomonas aeruginosa PAO1)
Geranoyl-CoA carboxylase. [EC: 6.4.1.5]

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4, 6.4.1.5

Use Curated BLAST to search for 6.4.1.4 or 6.4.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZX43 at UniProt or InterPro

Protein Sequence (538 amino acids)

>Pf6N2E2_2409 Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) (Pseudomonas fluorescens FW300-N2E2)
MPVIESHLDPHCADFTRNHAAMLAGVEQLRQLEQAVLEKAAQAQGKFDQRGQLMPRQRLN
LLLDPGAPFLELASLAGYKLHDDKDGSQAGGGLIAGIGYVSGVRVLVVANNSAIKGGTIS
PSGLYKTLRLQQIAMENKLPVITLAESGGANLNYAAQIFVEGARCFANQARMSAMGLPQI
TVVHGSATAGGAYQPGLSDYVVVVRDKARLFLAGPPLLKAATGEVASEEELGGAQMHAQV
AGTAEYLAENDADGVRLAREILSLLPWNAQLPPQPERSWTEPLYPADELLGLVPDDPKTP
YDVQEIIARIADGSNFLAFKSEFDAQTICGHLHIRGHACGLIGNNGPITPQGASKAAQFI
QLCDQSRTPLLFLHNTTGFMVGTESERQGVIKHGAKMIQAVANARVPKLTVVVGGSYGAG
NYAMCGRGLDPRFIFAWPNSHTAVMGGAQAGKVLRMVTEATQIKNGLTPDPKVLDLLEQT
TAQKLDSQSTALYGSASLWDDGLIDPRDTRTLLGYLLDICHEAEQRPLQANSFGVARF