Protein Info for Pf6N2E2_2329 in Pseudomonas fluorescens FW300-N2E2

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 586 TIGR00229: PAS domain S-box protein" amino acids 5 to 123 (119 residues), 57.5 bits, see alignment E=1.5e-19 PF13426: PAS_9" amino acids 17 to 120 (104 residues), 67.8 bits, see alignment E=3.1e-22 PF08448: PAS_4" amino acids 17 to 123 (107 residues), 36.6 bits, see alignment E=1.7e-12 PF00989: PAS" amino acids 18 to 104 (87 residues), 42.7 bits, see alignment E=1.8e-14 PF08447: PAS_3" amino acids 29 to 114 (86 residues), 53.2 bits, see alignment E=1e-17 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 128 to 292 (165 residues), 148 bits, see alignment E=2.1e-47 PF00990: GGDEF" amino acids 134 to 289 (156 residues), 158.8 bits, see alignment E=3.5e-50 PF00563: EAL" amino acids 309 to 544 (236 residues), 241 bits, see alignment E=4.2e-75

Best Hits

KEGG orthology group: None (inferred from 98% identity to pba:PSEBR_a1711)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZVI6 at UniProt or InterPro

Protein Sequence (586 amino acids)

>Pf6N2E2_2329 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas fluorescens FW300-N2E2)
MTASMDKKYRRAVDAAAIFSETDLSGRITYVNDQFCSLFGYRRDELLGVNHRLLNSGQHP
DEFFSSMWHTIALGQVWKGEICNRAKDGTLYWVDTTLVPVIDDDTGQVERYLAIRFDVSE
KRRLLHSLQWRVGHDVLTGLPNRTYLSDLLDQALEFSRAENLPLAVCMLDLDGFKAVNDG
YGHASGDRLLVEVARRLRSIVRGEDVVARLGGDEFVLVLRHVRGMDELHAALSRVLIAVS
MPYGIDGKGIKVFASIGVTLFPWDNEDAETLLRHADQAMYVAKQSGRNRFHLFDVSRDKE
VRATYQTVERVRQALAGNELRLHFQPKVNMRHGTVVGLEALLRWKHPQRGLVPPREFLPL
VEETDLIVEIGEWVMEQVLTQLQQWLQVGQGWPVSINISARHFQRADFVERLRQVLERHP
AVPPRLLDLQIVESVAVENLAHVSACLQACHALGVGFSLGGFGTGYCSLNDLKHLRTQTI
KIDKTFVRDILHDRDDLALTKAVIGLARAFGREVVAEGLDSLEHGQLLLNLGCEVAQGYF
IARPMPPERIPGWVQGFIPPSQWQQRPGDQGEGASLLACDASLRPV