Protein Info for Pf6N2E2_2260 in Pseudomonas fluorescens FW300-N2E2

Annotation: Serine phosphatase RsbU, regulator of sigma subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 transmembrane" amino acids 215 to 225 (11 residues), see Phobius details PF00072: Response_reg" amino acids 9 to 110 (102 residues), 104.6 bits, see alignment E=3.4e-34 PF07228: SpoIIE" amino acids 200 to 391 (192 residues), 115.3 bits, see alignment E=3.6e-37

Best Hits

KEGG orthology group: None (inferred from 100% identity to pba:PSEBR_a1776)

Predicted SEED Role

"Serine phosphatase RsbU, regulator of sigma subunit" in subsystem SigmaB stress responce regulation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZWS4 at UniProt or InterPro

Protein Sequence (393 amino acids)

>Pf6N2E2_2260 Serine phosphatase RsbU, regulator of sigma subunit (Pseudomonas fluorescens FW300-N2E2)
MPKTSATLLIIDDDEVVRASLAAYLEDSGFSVLQASNGQQGLQVFEQDKPDLVICDLRMP
QMGGLELIRQVTEISSQTPVIVVSGAGVMNDAVEALRLGAADYLIKPLEDLAVLEHSVRR
ALDRSRLLVENQRYREKLETANRELEASLNLLQEDQNAGRQVQMNMLPVSPWTVDDFRFA
HQIIPSLYLSGDFVDYFRVDERRVAFYLADVSGHGASSAFVTVLLKFMTTRLLFESKRNG
TLPEFKPSEVLGHINRGLISCKLGKHVTMVGGVIDEETGLLTYSIGGHLPLPVLYTPDSV
RYLEGRGLPVGLFNEATYEDHVLELPPTFSLTLMSDGILDLLTEPTLKEKEAALPERVSA
AGGSLEGLRQVFGLATLGEMPDDIALLVLSRNL