Protein Info for Pf6N2E2_1961 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): ABC transporter for D-sorbitol, permease component 2
Rationale: Specifically important in carbon source D-Sorbitol. We do not have fitness data for the putative first subunit Pf6N2E2_1962.
Original annotation: Various polyols ABC transporter, permease component 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 277 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 75 to 98 (24 residues), see Phobius details amino acids 110 to 130 (21 residues), see Phobius details amino acids 142 to 164 (23 residues), see Phobius details amino acids 196 to 217 (22 residues), see Phobius details amino acids 240 to 262 (23 residues), see Phobius details PF00528: BPD_transp_1" amino acids 91 to 264 (174 residues), 56 bits, see alignment E=2.3e-19

Best Hits

KEGG orthology group: K10229, sorbitol/mannitol transport system permease protein (inferred from 99% identity to pba:PSEBR_a2026)

MetaCyc: 90% identical to polyol ABC-type transporter permease component MtlG (Pseudomonas fluorescens)
7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]; 7.5.2.M2 [EC: 7.5.2.M2]

Predicted SEED Role

"Various polyols ABC transporter, permease component 2" in subsystem Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZW38 at UniProt or InterPro

Protein Sequence (277 amino acids)

>Pf6N2E2_1961 ABC transporter for D-sorbitol, permease component 2 (Pseudomonas fluorescens FW300-N2E2)
MMTLKQSRSLQSLLLGTLAWVAALLLFFPIFWMVLTSFKTEIDAFATPPQFIFMPTLENY
LHIQERSDYFHFAWNSVLISFSATALCMLIAVPAAYSMAFYETKRTKQTLLWMLSTKMLP
PVGVLMPIYLLAKGAGLLDTRIALIVIYTLINLPIVVWMIYTYFKDIPREILEAARLDGA
TLGQEMLRVLLPISKGGLASTMLLSMILCWNEAFWSLNLTSSSAAPLTALIASYSSPEGL
FWAKLSAVSTLACAPILIFGWISQKQLVRGLSFGAVK