Protein Info for Pf6N2E2_1939 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 811 transmembrane" amino acids 23 to 47 (25 residues), see Phobius details amino acids 53 to 70 (18 residues), see Phobius details amino acids 77 to 94 (18 residues), see Phobius details amino acids 102 to 121 (20 residues), see Phobius details amino acids 138 to 158 (21 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 27 to 808 (782 residues), 837.9 bits, see alignment E=3.1e-256 PF13360: PQQ_2" amino acids 220 to 319 (100 residues), 27.6 bits, see alignment E=3.3e-10 amino acids 373 to 471 (99 residues), 22.3 bits, see alignment E=1.4e-08 amino acids 684 to 790 (107 residues), 28.2 bits, see alignment E=2.3e-10 PF01011: PQQ" amino acids 246 to 275 (30 residues), 24.9 bits, see alignment (E = 1.9e-09) amino acids 371 to 396 (26 residues), 22.8 bits, see alignment (E = 8.5e-09) amino acids 454 to 479 (26 residues), 23.7 bits, see alignment (E = 4.6e-09) amino acids 735 to 772 (38 residues), 21.7 bits, see alignment 1.9e-08

Best Hits

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 99% identity to pba:PSEBR_a2046)

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.2

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZW22 at UniProt or InterPro

Protein Sequence (811 amino acids)

>Pf6N2E2_1939 Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2) (Pseudomonas fluorescens FW300-N2E2)
MTPIGAEPDSLNAKRDTTKASRWVCRIFAIVLLMLGLALSIGGIILVADAGSAYYLITGL
AFITSAVLLWRGDARGIWVYAAMLVWTTAWSLWEVGFNGWQLAPRLIGPFVLGAVLLLPH
FTRLKPAPSTRKLPRGWPSFAGGLVAAIALGSVSHAFGPDAPDFPMLRRGVQSQAPAKLP
EPLANNERTDWQAYGNDQGGTRFSPLADIDTTNVSKLVKVWEADMAPVNGDLNAIEGTPI
MMGSSLYACDGNNGIHAFDAETGKELWRRDISNGVPQSGKPCRGVAYYKVPDANGFCSER
IYAPSHNPTLVALDAKTGEYCPGFGNNGAVDLTKGVAPYPHGLFYVSSAPQVIRGKIVVG
GGIPDGQYWGGPSGVIRAFDAVTGELAWAFDAGAPNRLGLPPEGQYYTPSTPNSWAPISA
DEKLGLVYLPVGNATPDAYGGQRRPYDEDISSAVIALDAETGRLRWKFQATHHDIWDYDV
ASQPTLLNWPTAKGMRPALIQPTKRGEIFVLDRETGEPIKAVEEQPVPQRDIAKGEWLSP
TQPASVELPAFRGPQLREKDMWGATPIDQMVCRIMFKQSRYEGQFTPITLDKNVLIDPGS
MGGVNWNGISLDVDRGLMIVNWTQVPDRIELVTREEATQRNFRIAPGLDAGGQAEQPMLD
TPYGAYRVNFLSQLGIPCNAPPWGLIGAVDLVSGKLIWSKPFGSPRDIGPFGLPTLVSIP
IGTPTAGGAVTTRGGLVFIGGAAEHTFRALDAATGRELWSSRLATSANATPMTYRSPVSG
RQFVVVAEGGRPHYRTKPGSKLVAFAIPDSK