Protein Info for Pf6N2E2_1904 in Pseudomonas fluorescens FW300-N2E2

Annotation: Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 transmembrane" amino acids 27 to 48 (22 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 22 to 42 (21 residues), 16.8 bits, see alignment (E = 3.4e-07) PF20256: MoCoBD_2" amino acids 37 to 192 (156 residues), 46.1 bits, see alignment E=4.9e-16 amino acids 609 to 696 (88 residues), 47.2 bits, see alignment E=2.2e-16 PF02738: MoCoBD_1" amino acids 342 to 574 (233 residues), 105.7 bits, see alignment E=2.1e-34

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 99% identity to pba:PSEBR_a2076)

MetaCyc: 70% identical to 2-deoxy-D-ribose dehydrogenase beta subunit (Pseudomonas simiae)
1.1.2.M2 [EC: 1.1.2.M2]

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.1.2.M2 or 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9WH74 at UniProt or InterPro

Protein Sequence (752 amino acids)

>Pf6N2E2_1904 Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16) (Pseudomonas fluorescens FW300-N2E2)
MSKLDDSIAERGVLSDAQPVNVSRRRFLQASAGVTAGALVISFGIPLGPLRAAERAQALP
PGARVPAFLEIRPDNTVRLQSPFIEGGQGIFTGMAQVVGEELDIDPASFLVENAAPGNDF
KVMEGGMRFTGGSRSMRASYMTMRRLGASARLMLLQAASSHWKVPLAQLTTEPGRVIHAA
SNRSITYGELAPLTLDLPIPDPQTVTLRDPATFRWIGKPVQRIDVYDKSTGKAQYTIDTR
VEGMLHAAVQHAPRLGLQVGLVRNEDQIRNLKGVHSVHRLEGAVAVVAQRWWVAKRAVEA
LQVEWTEPGGDSTVRYMPANFDTQAYAEQLSKTTDAGDEAEREGDLQQGLAGAKKVVSAT
YHTQYLNHAQLEPPSALARFDAEGNLDLWLPNQAPDLFLADVAKRTGLDPSKITLHSPLL
GGFFGRHFLYPNASPYPQAIQLAQSTGRPIKVIWSREEEFLRDVLRPSAVVRFRAGLDEQ
GMPVAFEAISATEGPTEGIANKQGDKIDPTAVEGLAGKKYAIAHRRIAQLYSQTPVMLGY
WRSVGNSINDFMYEAFLDEVADAGGQDPYELRAKLLQGNERLSHLLKVVADLSGGWKRGP
FTAQDGTKRARGVAMASPFGTETAVIAEVSISNGAVKVHDIWQAIDPGSIVNPAIIEAQV
NSAVALGLSQTLVEQAIYKGGTPVARNYDLYRILTPEMMARVHVRVVESGAPMGGIGEPP
LPAVAPAVANAVSRLTGQRIRSMPLSQYRFDT