Protein Info for Pf6N2E2_1879 in Pseudomonas fluorescens FW300-N2E2

Annotation: Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 511 PF00501: AMP-binding" amino acids 11 to 371 (361 residues), 282.1 bits, see alignment E=6.4e-88 PF13193: AMP-binding_C" amino acids 422 to 497 (76 residues), 69 bits, see alignment E=5.7e-23

Best Hits

KEGG orthology group: None (inferred from 61% identity to bph:Bphy_4871)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZYA8 at UniProt or InterPro

Protein Sequence (511 amino acids)

>Pf6N2E2_1879 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (Pseudomonas fluorescens FW300-N2E2)
MEHSIGNWVGRSAARFGNKPAIIFEGKYWSFLDIDVQSSRLAASLESMGVKREDVVSIYS
PNSPEWIITYYAILKIGAIVNPLNTMLTAREAAFAIKNCGAVAVFSTSDKLLALQEHIGP
TEVISLISFDGITVAGMRHFNLLVDANVAVREYPVTGIQKDDISTIGYTSGTTGQPKGAV
LSHRCILTNVSMTATMHLRTASDIAVSALPLSHVYGNVVMNSAIAYGMTLVLHKTFDAEA
ILSSIQIYGATLLEGVPTMYIYLLNCPNLGAYDVSSLTRCTVGGQMMPYTAMENVERALG
CRLLELWGMTELGGLGTTHSLYGERRLGSIGVALPHLEARIAQLELDGDALPLGEIGELQ
IRGPVVMKHYLGRPDATAETKTDEGWLRTGDLARMDSEGFIYIVDRLKDMFITAGFNIYP
AELERVIAEHPSVAMVAVGSVLDEIKGELAKAYIVPKTGYEIDIQQIERHCRDRLAAYKV
PRLFQIVEDLPKTSSGKVMRRMLRQIFESKL