Protein Info for Pf6N2E2_1789 in Pseudomonas fluorescens FW300-N2E2

Annotation: Kup system potassium uptake protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 613 transmembrane" amino acids 38 to 61 (24 residues), see Phobius details amino acids 92 to 113 (22 residues), see Phobius details amino acids 130 to 148 (19 residues), see Phobius details amino acids 157 to 180 (24 residues), see Phobius details amino acids 193 to 217 (25 residues), see Phobius details amino acids 237 to 257 (21 residues), see Phobius details amino acids 277 to 302 (26 residues), see Phobius details amino acids 328 to 348 (21 residues), see Phobius details amino acids 354 to 380 (27 residues), see Phobius details amino acids 386 to 404 (19 residues), see Phobius details amino acids 410 to 431 (22 residues), see Phobius details PF02705: K_trans" amino acids 2 to 452 (451 residues), 615 bits, see alignment E=6.8e-189 PF22776: K_trans_C" amino acids 466 to 613 (148 residues), 171.4 bits, see alignment E=1.2e-54

Best Hits

Swiss-Prot: 83% identical to KUP_PSEF5: Probable potassium transport system protein kup (kup) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03549, KUP system potassium uptake protein (inferred from 100% identity to pba:PSEBR_a2233)

MetaCyc: 52% identical to K+:H+ symporter Kup (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-3

Predicted SEED Role

"Kup system potassium uptake protein" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZWC9 at UniProt or InterPro

Protein Sequence (613 amino acids)

>Pf6N2E2_1789 Kup system potassium uptake protein (Pseudomonas fluorescens FW300-N2E2)
LLVAAVGVVYGDIGTSPLYTLKEVFAGHYGVQANQDGVLGILSLIFWSLIWVVSIKYVLF
ILRANNQGEGGIMALTALARRAAAPFPKMSRVLVLLGLFGAALFYGDSMITPAISVLSAV
EGLQLAFDGIEQWVVPLSVVILVALFLIQKHGTARIGILFGPVMVLWFMVLGALGIYGIL
QRPEVLQALNPAWAVQFFVVHPGIGVAILGAVVLALTGAEALYADMGHFGRKPIARAWFI
LVLPGLVLNYFGQGALILGNPETVRNPFYLLAPEWALLPMVALSTLATIIASQAVISGAF
SLTRQAIQLGYVPRMFIQHTSSQEQGQIYIGTVNWALMVGVVLLVIGFESSSALAAAYGV
AVTGTMLITTVLSSAVVLLLWKTPRWLAVPMLLGFLLVDGLYFAANAPKIFQGGAFPVIA
GISLFILMTTWKRGRKIIVERLDETALPLPLFIGSIRTQPPHRVQGTAVFLTARTDAVPH
ALLHNLLHNQVLHEQVVLLTVVSEDSPRVSADRRFEIEAYGEGFFRVSLHFGFIEEPDVP
LALSLCHLAELDFSPMRTTYFLSRETVIPTKRIGMARWRETLFAFLLKNANSNLKYFKLP
LNRVIELGTQVEM