Protein Info for Pf6N2E2_1661 in Pseudomonas fluorescens FW300-N2E2

Annotation: RND efflux system, inner membrane transporter CmeB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1031 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 339 to 358 (20 residues), see Phobius details amino acids 365 to 387 (23 residues), see Phobius details amino acids 393 to 412 (20 residues), see Phobius details amino acids 434 to 457 (24 residues), see Phobius details amino acids 472 to 496 (25 residues), see Phobius details amino acids 539 to 557 (19 residues), see Phobius details amino acids 867 to 886 (20 residues), see Phobius details amino acids 894 to 913 (20 residues), see Phobius details amino acids 919 to 942 (24 residues), see Phobius details amino acids 968 to 988 (21 residues), see Phobius details amino acids 997 to 1021 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1021 (1021 residues), 1201.8 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1027 (1027 residues), 1511.7 bits, see alignment E=0 PF03176: MMPL" amino acids 346 to 497 (152 residues), 33.3 bits, see alignment E=2.6e-12

Best Hits

Swiss-Prot: 50% identical to TTGH_PSEPT: Toluene efflux pump membrane transporter TtgH (ttgH) from Pseudomonas putida (strain DOT-T1E)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 64% identity to atu:Atu3202)

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZXV1 at UniProt or InterPro

Protein Sequence (1031 amino acids)

>Pf6N2E2_1661 RND efflux system, inner membrane transporter CmeB (Pseudomonas fluorescens FW300-N2E2)
MPQFFIDRPVFAWVVALFILLAGILAIPQLPVAQYPVVAPPQIEIYTVYPGASAQTVDES
VVSLIEEELNGADNLLYFESQSSLGSATITATFQPGTNPELAQVDVQNRLKAVESRLPQA
VTQQGLQVDKVSAGFLLLITLTSSDGKLDDVALSDYLARNVMNEIKRLDGVGKAQLYGAE
RAMRIWIDPQKLVGFNLTPADVNAAIVAQNAQVSAGSLGDLPSRHTQEITATIVVKGQLN
TPQEFADIVLKANPDGSTVRIADVARVEIGSQEYQFSTRLNGKPSTAVAVQLSPGANALN
TATLVRNKMDELSHYFPAGVEYKIPYDTSPFVKVSITKVIYTLGEAMLLVFAVMFLFLQN
IRYTLIPTLVVPVALMGTFATMLALGFSINVLTMFGMVLAIGILVDDAIVVVENVERIMA
REGLSPKEATRKAMGQITGAIIGITLVLVAVFIPMAFMHGSVGVIYQQFSLSMATSILFS
AFLALTLTPALCATLLKPLAQGDHHAKTGFFGMFNRGFDRFGERYQGWVAYAVKRSGRYL
LIYGVLLVGMGVLFSRLPSSFLPVEDQGYTITDIQLPPGASKNRTVQVVEQIEAHNAGEP
GVGDSTIILGFSFSGSGQNAALAFTTLKDWSQRGAEDSAASIADRANLALGQIKDAMAFS
VLPPPVDGLGTSSGFEFRLQDRGGLGHATLMQARSELLAAADKSPMLMNVRESALAEAPQ
VQLEVDRKQANALGISFADVGSVLSTAVGSAYINDFPNQGRMQRVVVQAEGDQRSQVEDL
LKIHVRNNSGKMVPLSAFVQARWTQGPAQLTRYNGYPAVSISGEPAPGYSTGQAMAEIER
LVALGPTGLGQEWTGLSLQERLSGSQAPILLGLSLLVVFLCLAALYESWSIPTSVLLVVP
LGVLGAVLAVSLRGMPNDVFFKIGLITIIGLSAKNAILIIEFAKSLYDEGHDLIDATLQA
ARLRLRPIIMTSLAFILGVVPLAIATGASSASQQAIGTGVIGGMITATLAVVFVPVFFVV
VMKRVRRVEPK