Protein Info for Pf6N2E2_1306 in Pseudomonas fluorescens FW300-N2E2

Annotation: Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 301 TIGR02297: 4-hydroxyphenylacetate catabolism regulatory protein HpaA" amino acids 7 to 296 (290 residues), 436.3 bits, see alignment E=2.5e-135 PF02311: AraC_binding" amino acids 35 to 133 (99 residues), 53.2 bits, see alignment E=5.4e-18 PF07883: Cupin_2" amino acids 40 to 100 (61 residues), 30.5 bits, see alignment E=4.7e-11 PF12833: HTH_18" amino acids 217 to 296 (80 residues), 65.6 bits, see alignment E=8.1e-22 PF00165: HTH_AraC" amino acids 264 to 295 (32 residues), 28.4 bits, see alignment 2.8e-10

Best Hits

KEGG orthology group: K02508, AraC family transcriptional regulator, 4-hydroxyphenylacetate 3-monooxygenase operon regulatory protein (inferred from 99% identity to pba:PSEBR_a2745)

Predicted SEED Role

"Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZX11 at UniProt or InterPro

Protein Sequence (301 amino acids)

>Pf6N2E2_1306 Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family (Pseudomonas fluorescens FW300-N2E2)
MTDRQPIPNINIGQVYDQRYSDAEVHYDRLANLADFFGRNMPVHRHDRFFQVHYVKSGTV
RVYLDDQQYVESGPMFFLTPPTIPHSFVTEADSDGHVLTVRQQLVWQLIDADPGLAPAGV
QMPAACVALAQLGPGQAGEVRRLEYLFEELSEEVTAGRPGRSATLDALTRLIMISLLRLC
SNSLEARPARHEDLKIFHRFNELIEAHYLQHWPLSRYAEGIGVTEARLNDVCRRIADLPS
KRLIMERLMQEAKRLLLFTGSSANEICYQLGFKDPAYFSRFFQRYAQLTPGEYRQRQSGL
R