Protein Info for Pf6N2E2_1077 in Pseudomonas fluorescens FW300-N2E2

Annotation: Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 586 transmembrane" amino acids 387 to 405 (19 residues), see Phobius details TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 7 to 537 (531 residues), 677.6 bits, see alignment E=5.5e-208 PF00128: Alpha-amylase" amino acids 110 to 199 (90 residues), 49 bits, see alignment E=5.6e-17 PF11941: DUF3459" amino acids 502 to 570 (69 residues), 44.3 bits, see alignment E=1.7e-15

Best Hits

KEGG orthology group: K01236, maltooligosyltrehalose trehalohydrolase [EC: 3.2.1.141] (inferred from 98% identity to pba:PSEBR_a3007)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZUU7 at UniProt or InterPro

Protein Sequence (586 amino acids)

>Pf6N2E2_1077 Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141) (Pseudomonas fluorescens FW300-N2E2)
MLDEGHTRFALWAPDAFFVSVELDTGDSIPLLPQADGWFMIQTRCPAGTRYRYNIDGELE
VPDPASRAQAGDIDRHSVVVDPHAYSWRHTQWSGRPWHEAVIYELHVGALGGFDGVEQHL
ARLAGLGITAIELMPLAQFPGDRNWGYDGVLPYAPQASYGTPEQLKHLIDSAHGHGLSVI
LDVVYNHFGPDGNYLHRYAKGFFREDKHTPWGAAIDFRRREVRDFFIDNALMWLLEYRFD
GLRLDAIHAIEDPDFLQELATRVREQVDPIRHVWLTVENEHNQAHLLEHVYDAQWNDDGH
NALHVLLTGETDAYYADYAEQPTEQLARCLSQGFVFQGHLNRHGEARGEPSGHLPPSAFV
LFLQNHDQIGNRALGERLHQLAAPQALQAATVLLLLSPMIPLLFMGDEVAAEQPFLFFTS
HHGELAERVREGRRNEFKAFTAFADPQKRERIPDPNAASTFEASRPSLESQGPEQQANEA
LYRQLLKIRREEIVPRLPGAQALGTDVLGHGAISARWRLGDGSVLRIDFNLSDQPVEHTA
PDEARILFEHPQQVMGLLQQGVLAPYSALVSLTQATTLPNITGERL