Protein Info for Pf6N2E2_1041 in Pseudomonas fluorescens FW300-N2E2

Annotation: Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02515: CoA_transf_3
  • amino acids 13 to 374 (362 residues), 376.5 bits, E=7.5e-117

Best Hits

KEGG orthology group: None (inferred from 71% identity to aci:ACIAD2821)

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• ketogenesis (4/5 steps found)
• mevalonate degradation (1/2 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.4

Use Curated BLAST to search for 4.1.3.4

Sequence Analysis Tools

See A0A159ZSK2 at UniProt or InterPro

Protein Sequence (399 amino acids)

>Pf6N2E2_1041 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) (Pseudomonas fluorescens FW300-N2E2)
MSLHNENLQSLPLAGVRVIEFVHMVMGPTCGLVLADLGADVIKVEPVPEGDNTRRLTGSG
AGYWMTYNRNKKSFAVDIKTEEGMAAVCKLIESADVVTENFRPGAMEKLGLGYEQVKAIK
PDIIYSSMKGFLPGPYEHRTALDEVVQMMTGLAYMTGPEGRPLRAGASVNDVMGGMFSAI
SILAALWQRKNTGEGQFVQTGLFENSAFLVGQHMMQMATTGKAAAPMPSRLSAWAIYDVF
NTCDDEQVFMGVVSDSQWASFCQTFGFEELGRDPELAKNTQRVQARTRILPQVKERLAQF
SKQKVMAMCEQAGLPFSPIQRPQDLFDDQHLNESGGLAHLALESGEQVKVPMLPFEMNGR
RFDTRLNVPQLGSHSTELLEEMGYASGDVDALRARGIIR