Protein Info for Pf6N2E2_1033 in Pseudomonas fluorescens FW300-N2E2

Annotation: ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF13379: NMT1_2" amino acids 65 to 265 (201 residues), 36.5 bits, see alignment E=7.6e-13 PF12974: Phosphonate-bd" amino acids 73 to 177 (105 residues), 34.6 bits, see alignment E=2.1e-12 PF09084: NMT1" amino acids 83 to 204 (122 residues), 37.6 bits, see alignment E=3.4e-13

Best Hits

KEGG orthology group: None (inferred from 53% identity to avn:Avin_31380)

Predicted SEED Role

"ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components" in subsystem Alkanesulfonate assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165YYB9 at UniProt or InterPro

Protein Sequence (341 amino acids)

>Pf6N2E2_1033 ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components (Pseudomonas fluorescens FW300-N2E2)
MPRRTWKNTTALLTGAALLLAGCGESSDTKSPSAKAPDLSSVTLVLGDQAKGLRTVVEAS
NALEGIEYKVQWANFQGAAPLFEALRAGAVDLAPAGDTPVLAAATGGTPLRIVAVRRGQA
RGIAILVPPDSTIRSVADLKGRNVVVSSARGSIAQYLLIRALANAGVDENDVKVGFVLPT
DALPAFNAGKIEAWATFGVYQAFAEQKGARVLLSGEGINSGLTFITASDEVLNDPLKRKA
LSDVLGRFAKAFEWAQDNRDEYARVFAKVNDIPLEVSQTLRSWGDESLVLIEARDVQALQ
QVDDLFVEKKIFPHRVDVKALTNEQVFSPTPLVLTQSESAR