Protein Info for Pf6N2E2_1024 in Pseudomonas fluorescens FW300-N2E2

Annotation: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 transmembrane" amino acids 77 to 100 (24 residues), see Phobius details TIGR03860: FMN-dependent oxidoreductase, nitrilotriacetate monooxygenase family" amino acids 9 to 425 (417 residues), 547.9 bits, see alignment E=7.8e-169 PF00296: Bac_luciferase" amino acids 27 to 382 (356 residues), 169.4 bits, see alignment E=6.6e-54

Best Hits

Swiss-Prot: 55% identical to MOXC_BACSU: Putative monooxygenase MoxC (moxC) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 86% identity to pfs:PFLU3690)

MetaCyc: 55% identical to N-acetyl-S-alkylcysteine sulfoxide C-monooxygenase (Bacillus subtilis subtilis 168)
1.14.14.-

Predicted SEED Role

"Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases"

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZUQ9 at UniProt or InterPro

Protein Sequence (450 amino acids)

>Pf6N2E2_1024 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases (Pseudomonas fluorescens FW300-N2E2)
MSKQRQLKLGAMVHGVGHGWGEWRHPQAQPNASTDFGFYKHQTQLAEGAKFDFVFIADSL
HIHEKSSPHYLNRFEPLTILSALAALTSHIGLVATVTVSYTEPYQVARQFASLDHISGGR
AGWNVVTSWLSGTADNFGKSEHPPHAVRYRIAKEHVAVVKGLWDSWEDDAFTYDKQTGEF
FAPGKLHALEHKGEFFSVKGPLNIARSRQGQPVIFQAGTSEDGRNFAAENSDAIFVGAES
FEEAQAYYQDLKRRAKGFGRDAAQLSILPGIRPIVGRDEAEVEERYQQAVSLVSIEDAIV
ALGRPFNDHDFSQYPLDAPFPELGDLGANSQKGGSDRIKQLARDEGLTLREVALRFSQPR
SDFVGTPEQVADAIQTWFENGAADGFIINSLLPDGLQYFTELVVPVLQQRGLFRSEYSGH
TLRDNLGLQVPTNRYAQAHRSTEKTREAVA