Protein Info for Pf1N1B4_902 in Pseudomonas fluorescens FW300-N1B4

Annotation: Valyl-tRNA synthetase (EC 6.1.1.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 948 transmembrane" amino acids 501 to 523 (23 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 1 to 928 (928 residues), 1196 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 628 (615 residues), 512.2 bits, see alignment E=2.5e-157 PF09334: tRNA-synt_1g" amino acids 38 to 89 (52 residues), 22.1 bits, see alignment 1.5e-08 PF13603: tRNA-synt_1_2" amino acids 244 to 302 (59 residues), 28.4 bits, see alignment 2.8e-10 PF08264: Anticodon_1" amino acids 669 to 821 (153 residues), 147.3 bits, see alignment E=8e-47 PF10458: Val_tRNA-synt_C" amino acids 883 to 945 (63 residues), 67.6 bits, see alignment 2.4e-22

Best Hits

Swiss-Prot: 66% identical to SYV_COLP3: Valine--tRNA ligase (valS) from Colwellia psychrerythraea (strain 34H / ATCC BAA-681)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 66% identity to abc:ACICU_02995)

MetaCyc: 64% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MWL4 at UniProt or InterPro

Protein Sequence (948 amino acids)

>Pf1N1B4_902 Valyl-tRNA synthetase (EC 6.1.1.9) (Pseudomonas fluorescens FW300-N1B4)
MDKTYQPHAIETSWYNTWESENYFAPQGAGESYTIMIPPPNVTGSLHMGHGFNNAIMDAL
IRFRRMQGRNTLWQPGTDHAGIATQMLVERQLEAQGQNRHDLGREKFLEKVWEWKDQSGG
NISRQIRRLGSSVDWSRERFTMDDGLSEAVKEAFVRLHEDGLIYRGKRLVNWDTKLHTAI
SDLEVENHDEKGFLWNLKYPLADGAKTAEGKDYLIVATTRPETMLGDAAVAVNPNDERYK
ALIGKFVELPLVGRRIPIIADDYCDPEFGTGCVKITPAHDFNDYEVGKRHNLPLLNIFDK
NADVLPAAQVFNLDGSVNEQIDGTLPAEYVGLNRFQARKEIVAAFEAAGLLVSVDDHALK
VPKGDRSGTIIEPWLTDQWYVSTKPLAEPAIAAVEDGRIQFVPKQYENMYFSWMRDIQDW
CISRQLWWGHRIPAWYDESGKVYVGRDEAEVRAKNNLGPDVALQQDNDVLDTWFSSGLWT
FSTLGWPEQTEFLKKFHSTDVLVTGFDIIFFWVARMIMLTMHLVKNEDGTPQVPFKTVYV
HGLVRDGQGQKMSKSKGNVLDPLDIIDGIELEELVQKRTSGMMQPKLAKKIEKQTRDEFA
DGIASYGTDALRFTFCSLASTGRDIKFDMGRVEGYRNFCNKIWNAARYVLDKGEDCGQNG
EAYELSLADRWIISQLQRTEAEVTRQLDQFRFDLAAQALYEFIWNQYCDWYLELSKPVLW
DENAPVERQRGTRRTLVRVLEVALRLAHPFMPFITEEIWQRVAPLAGIEGKTIMLQPWPV
ANEARIDQGAEDDIEWLKGLMLGTRNIRGEMNIGPGKPLNLFLKNVSAEDQRRLTENEAL
LKKLARLESITVLKAGEEAPLSATALVGEMEVLVPMAGLIDKDAELARLDKEILRLQGEV
QRVGGKLSNAGFVDKAPAEVIEKERAKLAEAEQALGKLAEQHARIASL