Protein Info for Pf1N1B4_820 in Pseudomonas fluorescens FW300-N1B4

Annotation: Glucose-selective porin OprB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 445 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF04966: OprB" amino acids 73 to 445 (373 residues), 451.5 bits, see alignment E=1.6e-139

Best Hits

Swiss-Prot: 67% identical to PORB_PSEAE: Porin B (oprB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K07267, porin (inferred from 92% identity to pfo:Pfl01_4576)

Predicted SEED Role

"Glucose-selective porin OprB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MUT8 at UniProt or InterPro

Protein Sequence (445 amino acids)

>Pf1N1B4_820 Glucose-selective porin OprB (Pseudomonas fluorescens FW300-N1B4)
VANRKALNLMGGFTALGLATCIQAAPAFDSDSPWMLGDWNGTRTELSEKGYDFKVDYTGE
MGSNLHGGYDHDRTARYSDQFGFGTHLDLQKILGWDDAEFQLTITERNGNNISNDRINDP
RVGGFTSAQEVWGRGQTWRLTQMWYQQKFFDQKLDIKVGRFGEGEDFNSFPCDFQNLAFC
GSQVGNWVGDIWYNWPVSQWAMRVKYHLTPQLYAQVGAYEQNPSNLDRDNGFKLSGSGTQ
GAILPVELVWTPKFNGLPGEYRAGYYYSNAKATDVYKDSHGQPAALSGEAYRSASSKHGV
WLGIQQQLTSRASDHSRGLSVFANGTMHDKKTNAIDNYVQAGVVYKGLFDARARDDIGFA
LARVHVNPAYRKNAEATNQARAVFDYDDPSFLPPQDTEYSAELYYGVHVTNWLTVRPNLQ
YIRHPGGVDKVDDALIGGIKIQSSF