Protein Info for Pf1N1B4_684 in Pseudomonas fluorescens FW300-N1B4

Annotation: C4-dicarboxylate transport sensor protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 transmembrane" amino acids 24 to 43 (20 residues), see Phobius details amino acids 316 to 335 (20 residues), see Phobius details PF00512: HisKA" amino acids 378 to 442 (65 residues), 27.6 bits, see alignment E=2.4e-10 PF02518: HATPase_c" amino acids 487 to 594 (108 residues), 80 bits, see alignment E=1.9e-26

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 90% identity to pfo:Pfl01_4457)

Predicted SEED Role

"C4-dicarboxylate transport sensor protein"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QHB2 at UniProt or InterPro

Protein Sequence (600 amino acids)

>Pf1N1B4_684 C4-dicarboxylate transport sensor protein (Pseudomonas fluorescens FW300-N1B4)
MVWCQPNLQPVSYSMLPTSRTLRLSLYTLLIITGAALAATLAIRHAERQALEEDATRASQ
QLALYANSLHTLIERYRALPAVLALDPQLRAALNGEVSPEEQLALNRKLEKINGAAQSST
LELLDRTGLAVAASNWRLPSSYVGHNYGFRPYFSQTRTQGTGRFYAVGVTSGIPGYFLSS
AVTGDDGEFLGAMVVKLEFPELEREWRQGSDTLLVSDARGIIFIANQPGWRYRLLRPLSA
SDHTELKATRQYDKQPLTPLEYRSVRRFDDNSDLTRVNGPEGTADYLWESLPLATEGWTL
HLLRRPQVAFEDLRNAGLAAAGVWLAVVFLLLFLNQRWRLAKLRQRSREELERLVEERTR
DLRTAQDGLVQSAKLAALGQMSAALAHEINQPLTAQRMQLATLRLLLDHGRVEDAYKALK
PVDDMLTRMAALTGHLKTFARKSPSGLRERLDLATVVDQSLQLLDTRLRDENVSLVLHLT
RPAWVRGDAIRLEQVLINLLRNALDAMHDKPCKRLEIRLEADEQLWRLTVADNGGGIAEE
NLAQVFDPFFTTKAVGDGLGLGLAVSFAIVHEAGGRLSADNGENGAVFTLTLPIDLEVPA