Protein Info for Pf1N1B4_5759 in Pseudomonas fluorescens FW300-N1B4

Annotation: Nitric-oxide reductase subunit B (EC 1.7.99.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 63 to 81 (19 residues), see Phobius details amino acids 93 to 115 (23 residues), see Phobius details amino acids 141 to 162 (22 residues), see Phobius details amino acids 170 to 190 (21 residues), see Phobius details amino acids 202 to 228 (27 residues), see Phobius details amino acids 240 to 262 (23 residues), see Phobius details amino acids 268 to 295 (28 residues), see Phobius details amino acids 309 to 330 (22 residues), see Phobius details amino acids 349 to 368 (20 residues), see Phobius details amino acids 388 to 414 (27 residues), see Phobius details amino acids 435 to 457 (23 residues), see Phobius details PF00115: COX1" amino acids 15 to 444 (430 residues), 242.1 bits, see alignment E=5.7e-76

Best Hits

Swiss-Prot: 82% identical to NORB_PSEST: Nitric oxide reductase subunit B (norB) from Pseudomonas stutzeri

KEGG orthology group: K04561, nitric oxide reductase, cytochrome b-containing subunit I [EC: 1.7.2.5] (inferred from 94% identity to pba:PSEBR_a3167)

MetaCyc: 82% identical to nitric oxide reductase large subunit (Stutzerimonas stutzeri)
NITRIC-OXIDE-REDUCTASE-RXN [EC: 1.7.2.5]

Predicted SEED Role

"Nitric-oxide reductase subunit B (EC 1.7.99.7)" in subsystem Denitrification (EC 1.7.99.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.7

Use Curated BLAST to search for 1.7.2.5 or 1.7.99.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QXT1 at UniProt or InterPro

Protein Sequence (475 amino acids)

>Pf1N1B4_5759 Nitric-oxide reductase subunit B (EC 1.7.99.7) (Pseudomonas fluorescens FW300-N1B4)
MSMANPHLKFASQAVAKPYFVFALMLFLGQVLFGLIMGVQYVVGDFLFPIIPFNVARMVH
TNLLIVWLLFGFMGAAYYLIPEEADRELHSPKLAIILFWVFAAAGVLTILGYLLVPYAGL
AKLTHNELLPTMGREFLEQPTITKMGIVVVCLGFLYNIGMTLLKGRKTTVSMVMMTGLIG
LAVFFLFSFYNPGNLARDKFYWWWVVHLWVEGVWELIMGSMLAFVLIKITGVDREVVEKW
LYVIIAMALITGIIGTGHHFFWIGAPEVWLWVGSIFSALEPLPFLAMVIFAFSMVKNRRR
QHPNRAATLWAKGTTVTAFFGAGVWGFLHTLAPVNFYTHGSQLTAAHGHLAFYGAYAMIV
MTLISYAMPRLRGLGEAADERSQTLEIWGFWLMTLSMVMITLFLTAAGVVQVYLQRWQAD
GIALPFMATVEHLQVLFWARLGAGLGFFAGLLCYLLSFKQRSRAALRAPAAVVPS