Protein Info for Pf1N1B4_4255 in Pseudomonas fluorescens FW300-N1B4
Annotation: Putative phosphatase YfbT
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 41% identical to HXPA_ECOLI: Hexitol phosphatase A (hxpA) from Escherichia coli (strain K12)
KEGG orthology group: K01112, [EC: 3.1.3.-] (inferred from 75% identity to pfo:Pfl01_3528)MetaCyc: 41% identical to hexitol phosphatase A (Escherichia coli K-12 substr. MG1655)
Sorbitol-6-phosphatase. [EC: 3.1.3.50]; Sugar-phosphatase. [EC: 3.1.3.50, 3.1.3.23]; Mannitol-1-phosphatase. [EC: 3.1.3.50, 3.1.3.23, 3.1.3.22]; Glycerol-1-phosphatase. [EC: 3.1.3.50, 3.1.3.23, 3.1.3.22, 3.1.3.21]
Predicted SEED Role
"Putative phosphatase YfbT" in subsystem 2-phosphoglycolate salvage
MetaCyc Pathways
- mannitol cycle (4/5 steps found)
- D-sorbitol biosynthesis I (2/3 steps found)
- mannitol biosynthesis (2/3 steps found)
- 1,3-propanediol biosynthesis (engineered) (4/9 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Ether lipid metabolism
- Fructose and mannose metabolism
- Glycerolipid metabolism
- Lipopolysaccharide biosynthesis
- Nicotinate and nicotinamide metabolism
- Riboflavin metabolism
- Sphingolipid metabolism
- Thiamine metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.1.3.-
Use Curated BLAST to search for 3.1.3.- or 3.1.3.21 or 3.1.3.22 or 3.1.3.23 or 3.1.3.50
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166PX12 at UniProt or InterPro
Protein Sequence (222 amino acids)
>Pf1N1B4_4255 Putative phosphatase YfbT (Pseudomonas fluorescens FW300-N1B4) LSVQASVFNTSYRAFLFDMDGTVLNSIAAAERIWTTWALRHGVDVDTFLPTIHGVRAIDT ITRLALPGVDAEAEAAWITEAEIEDVEGIVEVAGAASFLKSLPANQWAIVTSAPRALALR RMAAAGIPEPLVMVTAEDVSAGKPDPAGYRLAARRLGVEPTECLIFEDAAVGIRAGEAAG AHVMVITATHVHPLQTPHATLAGYDSVEARVDLHSQISLYTL