Protein Info for Pf1N1B4_364 in Pseudomonas fluorescens FW300-N1B4

Annotation: Rhodanese domain protein UPF0176

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 PF17773: UPF0176_N" amino acids 6 to 96 (91 residues), 101.8 bits, see alignment E=2.2e-33 PF00581: Rhodanese" amino acids 120 to 211 (92 residues), 35.5 bits, see alignment E=1.1e-12

Best Hits

Swiss-Prot: 96% identical to Y4311_PSEFS: UPF0176 protein PFLU_4311 (PFLU_4311) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K07146, UPF0176 protein (inferred from 96% identity to pfs:PFLU4311)

Predicted SEED Role

"Rhodanese domain protein UPF0176"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AQN5 at UniProt or InterPro

Protein Sequence (313 amino acids)

>Pf1N1B4_364 Rhodanese domain protein UPF0176 (Pseudomonas fluorescens FW300-N1B4)
MTQQIVVAALYKFVTLEDYVALREPLLQAMVDNGIKGTLLIAEEGINGTVSGSREGIDGL
MAWLKNDPRMDDIDHKESYCDEQPFYRTKVKLKKEIVTLGVEGVDPNKKVGTYVDPQNWN
ALISDPEVLLIDTRNDYEVSIGTFEGAIDPKTTSFREFPDYIKAHFDPAVHKKVAMFCTG
GIRCEKASSYMLSQGFDEVYHLKGGILKYLEEVPQEETKWQGDCFVFDNRVTVRHDLSEG
DYDQCHACRTPVSVEDRASEHYVAGISCPHCWDKLSEKTRRSAIDRQKQIELAKARNLPH
PIGYNYKQTPSEA