Protein Info for Pf1N1B4_3156 in Pseudomonas fluorescens FW300-N1B4

Annotation: Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase RhlG (EC 1.1.1.100)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08659: KR
  • amino acids 11 to 159 (149 residues), 38.6 bits, E=2.2e-13
• PF00106: adh_short
  • amino acids 11 to 207 (197 residues), 174.4 bits, E=4e-55
• PF01370: Epimerase
  • amino acids 12 to 203 (192 residues), 21.3 bits, E=3.3e-08
• PF13561: adh_short_C2
  • amino acids 19 to 255 (237 residues), 189.5 bits, E=1.5e-59

Best Hits

Swiss-Prot: 75% identical to RHLG_PSEAE: Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (rhlG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 91% identity to pfo:Pfl01_1187)

MetaCyc: 75% identical to NADPH-dependent beta-ketoacyl reductase subunit (Pseudomonas aeruginosa)
3-oxoacyl-[acyl-carrier-protein] reductase. [EC: 1.1.1.100]

Predicted SEED Role

"Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase RhlG (EC 1.1.1.100)" in subsystem Rhamnolipids in Pseudomonas (EC 1.1.1.100)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
• palmitate biosynthesis III (28/29 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• anteiso-branched-chain fatty acid biosynthesis (30/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (30/34 steps found)
• tetradecanoate biosynthesis (mitochondria) (23/25 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
• octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (12/12 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• biotin biosynthesis I (13/15 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• fatty acid elongation -- saturated (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
• (5Z)-dodecenoate biosynthesis II (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• stearate biosynthesis IV (4/6 steps found)
• petroselinate biosynthesis (2/6 steps found)
• 2-allylmalonyl-CoA biosynthesis (2/8 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (20/205 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.100

Use Curated BLAST to search for 1.1.1.100

Sequence Analysis Tools

See A0A166P5B4 at UniProt or InterPro

Protein Sequence (256 amino acids)

>Pf1N1B4_3156 Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase RhlG (EC 1.1.1.100) (Pseudomonas fluorescens FW300-N1B4)
MHPYFSLAGRTALVTGGTRGIGKMIAKAYVEAGATVYVCARDADACQQTAKELSAFGVCH
AVAANLAHEEGVQQLAAQLSEQIGQLDILVNNAGTTWGAPLESYPVKGWEKVMQLNVTSV
FNCIQQFLPLLRKAGSAANPARIINIGSVAGISSFGEQAYAYGPSKAALHQLSRILAREL
VSQHINVNVIAPGRFPSKMTQHIGNDEQALAEDTALIPMKRWGREEEMAALAISLASMAG
AYMTGNIIPLDGGFSL