Protein Info for Pf1N1B4_1726 in Pseudomonas fluorescens FW300-N1B4

Annotation: Sulfate transport system permease protein CysT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 transmembrane" amino acids 12 to 37 (26 residues), see Phobius details amino acids 65 to 85 (21 residues), see Phobius details amino acids 97 to 119 (23 residues), see Phobius details amino acids 135 to 155 (21 residues), see Phobius details amino acids 187 to 206 (20 residues), see Phobius details amino acids 242 to 265 (24 residues), see Phobius details TIGR02139: sulfate ABC transporter, permease protein CysT" amino acids 8 to 270 (263 residues), 424.9 bits, see alignment E=1.3e-131 TIGR00969: sulfate ABC transporter, permease protein" amino acids 10 to 268 (259 residues), 313.5 bits, see alignment E=1.3e-97 PF00528: BPD_transp_1" amino acids 75 to 269 (195 residues), 75.4 bits, see alignment E=2.5e-25

Best Hits

Swiss-Prot: 50% identical to CYST_SALTY: Sulfate transport system permease protein CysT (cysU) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02046, sulfate transport system permease protein (inferred from 97% identity to pfl:PFL_0193)

MetaCyc: 49% identical to sulfate/thiosulfate ABC transporter inner membrane subunit CysU (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate transport system permease protein CysT" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166NCJ3 at UniProt or InterPro

Protein Sequence (272 amino acids)

>Pf1N1B4_1726 Sulfate transport system permease protein CysT (Pseudomonas fluorescens FW300-N1B4)
MSRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWEQFWAIISAPRVLAALK
LSFGTALYAAIINGIIGTLLAWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYTP
TGLVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQPVLADIPREVEEAAACLGAKP
LQVFRHILVPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
YTGATSIGVLMLVVSFVLLLLINLLQRRIETP