Protein Info for Pf1N1B4_1575 in Pseudomonas fluorescens FW300-N1B4

Annotation: Proline iminopeptidase (EC 3.4.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 TIGR01249: prolyl aminopeptidase" amino acids 9 to 310 (302 residues), 440.7 bits, see alignment E=1.4e-136 PF00561: Abhydrolase_1" amino acids 36 to 298 (263 residues), 130.7 bits, see alignment E=1.2e-41 PF12146: Hydrolase_4" amino acids 37 to 293 (257 residues), 48 bits, see alignment E=1.5e-16 PF12697: Abhydrolase_6" amino acids 37 to 295 (259 residues), 55.1 bits, see alignment E=2.7e-18

Best Hits

Swiss-Prot: 56% identical to PIP_LEPBY: Probable proline iminopeptidase (pip) from Leptolyngbya boryana

KEGG orthology group: K01259, proline iminopeptidase [EC: 3.4.11.5] (inferred from 95% identity to pba:PSEBR_a389)

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.5

Use Curated BLAST to search for 3.4.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162ASY3 at UniProt or InterPro

Protein Sequence (323 amino acids)

>Pf1N1B4_1575 Proline iminopeptidase (EC 3.4.11.5) (Pseudomonas fluorescens FW300-N1B4)
MQTLYPQIKPHARHDLAVDKTHTLYVDESGSPEGLPVVFIHGGPGAGCDAQSRRYFDPNL
YRIVTFDQRGCGRSTPHASLENNTTWDLVADLERIRKHLGIDKWVLFGGSWGSTLALAYA
QTHPERVHGLILRGIFLCRPQEIEWFYQCGASRLFPDYWQDYIAPIPQDERHDLLSAFHK
RLVGNDQIAQMHAAKAWSMWEGRTATLRPNPLVVDRFSEPQRALSIARIECHYFTNNAFL
EPNQLIRDMAKIAHLPGVIVHGRYDVICPLDNAWELHQAWPNSELQVIRDAGHAASETGI
TDALVRAADLMARRMLDLSPDEA