Protein Info for Pf1N1B4_1018 in Pseudomonas fluorescens FW300-N1B4

Annotation: Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 4 to 73 (70 residues), 64.3 bits, E=1.1e-21
• PF02817: E3_binding
  • amino acids 114 to 148 (35 residues), 52.3 bits, 7.9e-18
• PF00198: 2-oxoacid_dh
  • amino acids 161 to 368 (208 residues), 186.3 bits, E=9.5e-59

Best Hits

KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 82% identity to pmy:Pmen_3245)

Predicted SEED Role

"Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 2.3.1.168)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.12, 2.3.1.168

Use Curated BLAST to search for 2.3.1.12 or 2.3.1.168

Sequence Analysis Tools

See A0A166MYI1 at UniProt or InterPro

Protein Sequence (369 amino acids)

>Pf1N1B4_1018 Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168) (Pseudomonas fluorescens FW300-N1B4)
MKYFKLPDLGEGLQEAEIVRWHVKVGDTVTADQLLVSVETAKALVDIPAPYDGVVAKTFG
GEGDILHVGEPLLGYEGEADAGTVVGRLEDGGTNQEDAFFIGAAPSTREHLANRATPAVR
QLARQLGVELSGLTGSGQDGQITRSDVENAAQTERERFGGEKLRGVRRSMALNMARSHAE
VVPVTIFGDADLHRWGQAREPLIRLAKAMAAACAVEPVLNSAFDGKTQALKQHERLDLGI
AVDTPDGLFVPVLRDVGQRTAADLKEGVMRLRADVQARSIPPQEMMGATLTLSNFGTLFG
RYANPVVVPPQVAILAAGAIREEPVAVEGAVVVHPILPLSLTFDHRVVTGGEAARFFKAL
VEALEQPEV