Protein Info for PFLU_RS27990 in Pseudomonas fluorescens SBW25-INTG

Annotation: betaine-aldehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01804: betaine-aldehyde dehydrogenase
  • amino acids 10 to 477 (468 residues), 758.4 bits, E=1.2e-232
• PF00171: Aldedh
  • amino acids 20 to 479 (460 residues), 606 bits, E=2e-186

Best Hits

Swiss-Prot: 100% identical to BETB_PSEFS: NAD/NADP-dependent betaine aldehyde dehydrogenase (betB) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00130, betaine-aldehyde dehydrogenase [EC: 1.2.1.8] (inferred from 100% identity to pfs:PFLU5685)

MetaCyc: 78% identical to betaine aldehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Betaine-aldehyde dehydrogenase. [EC: 1.2.1.8]

Predicted SEED Role

"Betaine aldehyde dehydrogenase (EC 1.2.1.8)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.2.1.8)

MetaCyc Pathways

• choline-O-sulfate degradation (3/3 steps found)
• choline degradation I (2/2 steps found)
• glycine betaine biosynthesis I (Gram-negative bacteria) (2/2 steps found)
• glycine betaine biosynthesis II (Gram-positive bacteria) (2/2 steps found)
• glycine betaine biosynthesis III (plants) (2/3 steps found)
• β-alanine biosynthesis I (1/2 steps found)
• β-alanine biosynthesis IV (1/2 steps found)
• choline degradation IV (2/4 steps found)
• dimethylsulfoniopropanoate biosynthesis I (Wollastonia) (1/3 steps found)
• dimethylsulfoniopropanoate biosynthesis II (Spartina) (1/4 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.8

Use Curated BLAST to search for 1.2.1.8

Sequence Analysis Tools

See C3K3D2 at UniProt or InterPro

Protein Sequence (490 amino acids)

>PFLU_RS27990 betaine-aldehyde dehydrogenase (Pseudomonas fluorescens SBW25-INTG)
MARFDLQKLYIDGGYSDAGSDATFEAINPANGEVLAQVQRATKEDVERAVVSAEKGQKIW
AAMTAMERSRILRRAVDILRERNDELAALETLDTGKAFSETQYVDVVTGADVLEYYAGLV
PAIEGEQIPLRDTSFVYTRREPLGVVAGIGAWNYPIQIALWKSAPALAAGNAMIFKPSEV
TSLTTLKLAEIYTEAGVPAGVFNVLTGSGREVGTWLTEHPRIEKVSFTGGTDTGKKVMAS
ASSSSLKEVTMELGGKSPLIVFEDADLDRAADIAMMANFYSSGQVCTNGTRVFVPKHLQA
AFEAKIVERVARIRVGDPQDENTNFGPLVSFAHMESVLGYIAKGKEQGARLLCGGDRLTD
GDFAKGAYVAPTVFTDCTDEMTIVREEIFGPVMSILTYETEEEVIRRANDTDFGLAAGLV
TKDLNRAHRVIHQLEAGICWINAWGESDAKMPVGGYKQSGVGRENGISSLNNFTRIKSVQ
VELGDYASVF