Protein Info for PFLU_RS25490 in Pseudomonas fluorescens SBW25-INTG

Annotation: helix-turn-helix domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 PF14525: AraC_binding_2" amino acids 14 to 195 (182 residues), 130.1 bits, see alignment E=1.1e-41 PF12833: HTH_18" amino acids 241 to 320 (80 residues), 66.1 bits, see alignment E=4.3e-22

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU5193)

Predicted SEED Role

"benABC operon transcriptional activator BenR" in subsystem Benzoate degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K201 at UniProt or InterPro

Protein Sequence (328 amino acids)

>PFLU_RS25490 helix-turn-helix domain-containing protein (Pseudomonas fluorescens SBW25-INTG)
MSSQTIQRFDLEGARSWMSGICGPHRLATATPERLRFHHSANVFKSRATTLGVIEYGTDV
TIDIEDAEHFSSYSLSLPLVGEQELSKNGERLSSNRDQGVIISPNEHQVLAISGDCRKLQ
VVITRAAMSESLEGLLQRPIEAPLRFESVMDAVEGASASWWRMARYFIAELEHGSELYEQ
AAFTRDLESSLIKGLILAQPNNYSEELRDVLGVKLPHYLIRARQYIHDNAREAVHLEDLE
AAAGVSRFKLFDAFRKYFALSPMAYLKKYRLGAVRQEILEHGSTRTISEIALGWGFTHLG
RFSAEYRKLFDESPSQTLQRNDARRLRG