Protein Info for PFLU_RS23645 in Pseudomonas fluorescens SBW25-INTG

Annotation: glutathione transferase GstA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02798: GST_N
  • amino acids 2 to 74 (73 residues), 36.1 bits, E=2e-12
• PF13409: GST_N_2
  • amino acids 10 to 75 (66 residues), 42.2 bits, E=2.9e-14
• PF13417: GST_N_3
  • amino acids 15 to 78 (64 residues), 32.5 bits, E=2.7e-11
• PF14497: GST_C_3
  • amino acids 122 to 194 (73 residues), 25.1 bits, E=5e-09
• PF00043: GST_C
  • amino acids 127 to 189 (63 residues), 34.3 bits, E=7.1e-12

Best Hits

Swiss-Prot: 46% identical to GSTA_ECOLI: Glutathione S-transferase GstA (gstA) from Escherichia coli (strain K12)

KEGG orthology group: K00799, glutathione S-transferase [EC: 2.5.1.18] (inferred from 100% identity to pfs:PFLU4821)

MetaCyc: 46% identical to glutathione S-transferase GstA (Escherichia coli K-12 substr. MG1655)
Glutathione transferase. [EC: 2.5.1.18]

Predicted SEED Role

"Glutathione S-transferase (EC 2.5.1.18)" in subsystem Glutathione: Non-redox reactions (EC 2.5.1.18)

MetaCyc Pathways

• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• pentachlorophenol degradation (3/10 steps found)
• gliotoxin biosynthesis (2/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18

Use Curated BLAST to search for 2.5.1.18

Sequence Analysis Tools

See C3JZ55 at UniProt or InterPro

Protein Sequence (200 amino acids)

>PFLU_RS23645 glutathione transferase GstA (Pseudomonas fluorescens SBW25-INTG)
MKLYFAPHACSLAPHIVLRELALPFDLVRVDNQAKTTADGEDFLQINPKGYVAALRLDDG
QVLTEASAILQYLADLKPTVGLAPANGSWERVRLQEWLNFIATEVHGGLAVFFNDAIQGE
VRALFQATLFKRFAVLVQTLEHQDYLLGTAYSVADAYLFVVLRWAGFHAIDLGEWPALAA
FQQRIGARPAVIAALAAESR