Protein Info for PFLU_RS07345 in Pseudomonas fluorescens SBW25-INTG

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 transmembrane" amino acids 29 to 47 (19 residues), see Phobius details amino acids 55 to 78 (24 residues), see Phobius details amino acids 89 to 109 (21 residues), see Phobius details amino acids 118 to 142 (25 residues), see Phobius details amino acids 163 to 180 (18 residues), see Phobius details amino acids 191 to 208 (18 residues), see Phobius details amino acids 232 to 257 (26 residues), see Phobius details amino acids 276 to 294 (19 residues), see Phobius details amino acids 306 to 326 (21 residues), see Phobius details amino acids 338 to 357 (20 residues), see Phobius details amino acids 369 to 391 (23 residues), see Phobius details amino acids 402 to 420 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 20 to 222 (203 residues), 82 bits, see alignment E=4.3e-27 amino acids 223 to 414 (192 residues), 40.2 bits, see alignment E=2.1e-14 PF07690: MFS_1" amino acids 22 to 383 (362 residues), 97 bits, see alignment E=1.1e-31 TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 23 to 413 (391 residues), 446.3 bits, see alignment E=5.6e-138

Best Hits

Swiss-Prot: 74% identical to CITH_KLEPN: Citrate-proton symporter (citH) from Klebsiella pneumoniae

KEGG orthology group: K03288, MFS transporter, MHS family, citrate/tricarballylate:H+ symporter (inferred from 100% identity to pfs:PFLU1489)

MetaCyc: 67% identical to propane-1,2,3-tricarboxylate-proton symporter (Salmonella enterica enterica serovar Typhimurium str. LT2)
RXN1R65-49

Predicted SEED Role

"metabolite/H symporter, major facilitator superfamily (MFS)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4S1 at UniProt or InterPro

Protein Sequence (434 amino acids)

>PFLU_RS07345 MFS transporter (Pseudomonas fluorescens SBW25-INTG)
MTSPTRPNSAHSKVGAVFRVTSGNFLEQFDFFLFGFYATYIAAAFFPSANEFASLMMTFA
VFGAGFLMRPLGAIILGAYIDDVGRRKGLIVTLSIMASGTLLIVLVPGYHTIGLWAPLLV
LLGRLLQGFSAGAELGGVSVYLSEMATPGRKGFYTSWQSGSQQISIVVAAALGYGLNVWM
EPAVVADWGWRIPFAIGCVIIPFIFILRRSLQETEEFANRKHRPTMREVLATLAKNWTVV
IGGMLMVAMTTTAFYLITVYAPTFGKTVLQLSTSDALLVTLLVAVSNFVWLPIGGTLSDR
FGRKPVLIAMTALTVLTAYPALSYVVNSPSFAHMLETLLWFSFLYGMYNGAMIPALTEIM
PVEVRVAGFSLAYSLATAIFGGFTPAISTWFIHITEDKASPAYWLMFAALCALCSTLALY
RRANARGQVMQGAA