Protein Info for PFLU_RS05920 in Pseudomonas fluorescens SBW25-INTG

Annotation: LysR family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 transmembrane" amino acids 112 to 133 (22 residues), see Phobius details PF00126: HTH_1" amino acids 25 to 83 (59 residues), 59.5 bits, see alignment E=3.8e-20 PF03466: LysR_substrate" amino acids 111 to 311 (201 residues), 189.2 bits, see alignment E=9.2e-60 PF12727: PBP_like" amino acids 188 to 309 (122 residues), 29.4 bits, see alignment E=6.6e-11

Best Hits

Swiss-Prot: 43% identical to YEIE_ECOL6: Uncharacterized HTH-type transcriptional regulator YeiE (yeiE) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU1197)

Predicted SEED Role

"LysR family transcriptional regulator PA3398" in subsystem DNA-binding regulatory proteins, strays

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDQ8 at UniProt or InterPro

Protein Sequence (328 amino acids)

>PFLU_RS05920 LysR family transcriptional regulator (Pseudomonas fluorescens SBW25-INTG)
MAATISVNWIIKIRVIGYIDMRFTLRQLQVFVAVAQQESVSRAAGLLALSQSAASTSITE
LERQSSCQLFDRAGKRLSLNALGHQLLPQAVALLDQAKEIEDLLNGKSGFGSLAVGATLT
IGNYLATLLIGSFMQQHPESQVKLHVQNTAHIVHQVAHYEIDLGLIEGDCSHPDIEVQTW
VEDELVVFCAPQHHLAKRGMASMDELTHEAWILREQGSGTRLTFDQAMRHHRSTLNIRLE
LEHTEAIKRAVESGLGIGCISRLALRDAFRRGSLVPVETPDLDLARQFYFIWHKQKYQTS
AMREFLELCRAFTAGVQRSDEIVLPSIA