Protein Info for PFLU_RS03185 in Pseudomonas fluorescens SBW25-INTG

Annotation: type II and III secretion system protein family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 405 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF13629: T2SS-T3SS_pil_N" amino acids 41 to 109 (69 residues), 59 bits, see alignment E=3e-20 PF00263: Secretin" amino acids 201 to 362 (162 residues), 154.4 bits, see alignment E=2.3e-49

Best Hits

KEGG orthology group: K02280, pilus assembly protein CpaC (inferred from 100% identity to pfs:PFLU0647)

Predicted SEED Role

"Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K823 at UniProt or InterPro

Protein Sequence (405 amino acids)

>PFLU_RS03185 type II and III secretion system protein family protein (Pseudomonas fluorescens SBW25-INTG)
MSQRYAPLFMHMAWALMLSSLPVGMALAAPGNCSALGQLPAVVEVGEGLQQELQSPVAIT
RLAIGDPKIADVRVNGDRHFLLTGVASGATSLMVWTACASTPRQSMVFVKGRATSALTSL
SLSPSEDPLLPSQVQTDIRFVEVSRTKLKEASTRLLGTGKNFFLGSPNLLSPSEGVFKLP
VSTDSFNVGFGGGRVSAMINALERSGFAYTLARPSLVAMSGQSASFLAGGEVPVPVPSAG
SDTISIEYKEFGIRLTLTPTVIDRNRITLKVAPEVSELDYSNAVIIQGIQVPALTVRRTD
TSVSLADGESFVISGLISTNNRSTISKFPGLGDIPILGAFFRDSNISREEKELLMIVTPH
LVQPLAANAQLPSLPGEKLRSYDPNWFRLFFLENGNFDRRSGLSQ