Protein Info for PFLU_RS03170 in Pseudomonas fluorescens SBW25-INTG

Annotation: type II secretion system F family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 signal peptide" amino acids 5 to 5 (1 residues), see Phobius details amino acids 23 to 25 (3 residues), see Phobius details transmembrane" amino acids 6 to 22 (17 residues), see Phobius details amino acids 70 to 88 (19 residues), see Phobius details amino acids 91 to 110 (20 residues), see Phobius details amino acids 236 to 258 (23 residues), see Phobius details amino acids 270 to 289 (20 residues), see Phobius details PF00482: T2SSF" amino acids 127 to 252 (126 residues), 60.9 bits, see alignment E=6.3e-21

Best Hits

KEGG orthology group: K12510, tight adherence protein B (inferred from 100% identity to pfs:PFLU0644)

Predicted SEED Role

"Flp pilus assembly protein TadB" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7F8 at UniProt or InterPro

Protein Sequence (293 amino acids)

>PFLU_RS03170 type II secretion system F family protein (Pseudomonas fluorescens SBW25-INTG)
MTGPVLLLICLLLIGLSFWQFHHGLRKAQTARVLERLGNGQPEAQETTTTWTGLERMFQR
AGLGKPTDRLGLWASAWVIGALLGWLLADGLGLIAMIVGPPLALRVFIAWRYQQRVQRMV
EQLPQLLDHSVRSLKSGRTLADAVLGGVESIENPLRDAMGRVQRNVRMGVSLPDSVSDFA
EFYEQDEFRLFALGLKVNHRYGGNASELLENLIKMIREREQGARQLKAMTGETRMTAYVL
GGLPVLIVGYFLVVNPGYLMNMWHDGTGRIMLFGALAMDLGGTFIMWRMLRSV