Protein Info for MPMX20_03668 in Enterobacter sp. TBS_079
Annotation: Flap endonuclease Xni
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to XNI_ESCF3: Flap endonuclease Xni (xni) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)
KEGG orthology group: K01146, protein Xni (inferred from 96% identity to enc:ECL_04131)Predicted SEED Role
"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.7
Use Curated BLAST to search for 2.7.7.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (251 amino acids)
>MPMX20_03668 Flap endonuclease Xni (Enterobacter sp. TBS_079) MAVHLLIVDALNLIRRIHAVQGTPCKDTCLHALEQLIRHSEPTHVVAVFDDEARNSGWRH QRLPDYKAGRAPMPDDLHAEMPAIRAAFEQRGVPCWGAHGNEADDLAATLAVKVANAGHQ ATIVSTDKGYCQLLSPTVRIRDYFQKRWLDAPFIASEFGVSPEQLPDYWGLAGISSSKVP GVAGIGPKSAAQLLTDFQSLEGIYARLDEVPEKWRKKLETHKEMAFICRDVATLQTDLQL DGNLQQLRLER